| 产品编号 | bs-3875R |
| 英文名称 | Peroxiredoxin 1 Rabbit pAb |
| 中文名称 | 过氧化还原酶1抗体 |
| 别 名 | Peroxiredoxin-1; Peroxiredoxin 1; Prdx 1; Prdx1; PRDX1_HUMAN; Hbp23; Heme binding 23 kDa protein; Macrophage 23 Kd stress protein; Macrophage 23kDa stress protein; Macrophase stress protein 22kDa; Macrophase stress protein 23 kd; MSP23; Natural killer cell enhancing factor A; Natural killer cell-enhancing factor A; Natural killer enhancing factor A; NKEF A; NKEF-A; NkefA; OSF3; Osteoblast specific factor 3; PAG; Paga; PAGB; Proliferation associated gene A; Proliferation associated protein PAG; Proliferation-associated gene protein; PrxI; Tdpx 2; Tdpx2; TDX2; Thioredoxin dependent peroxide reductase 2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; TPxA; Trx dependent peroxide reductase 2. |
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Specific References (3) | bs-3875R has been referenced in 3 publications.
[IF=2.86] Fu, Qiang, et al. "Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development."International Journal of Molecular Sciences 17.5 (2016): 618. WB ; Other Species.
[IF=2.21] Nedret Kilic. et al. Thioredoxin system and miR-21, miR-23a/b and let-7a as potential biomarkers for brain tumor progression: Preliminary Case Data. WORLD NEUROSURG. 2022 Sep;: WB ; Human.
[IF=2.15] Sun, Qi-Kai, et al. "Diagnostic and prognostic significance of peroxiredoxin 1 expression in human hepatocellular carcinoma." Medical Oncology 31.1 (2014): 1-9. IHC-P ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRDX1: 1-100/199 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]. Function: Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. Subunit: Homodimer; disulfide-linked, upon oxidation. May form heterodimers with AOP2. Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity). Subcellular Location: Cytoplasm. Melanosome. Post-translational modifications: Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. Similarity: Belongs to the AhpC/TSA family. Contains 1 thioredoxin domain. SWISS: Q06830 Gene ID: 5052 Database links: Entrez Gene: 5052 Human Entrez Gene: 18477 Mouse Omim: 176763 Human SwissProt: Q9JKY1 Chinese Hamster SwissProt: Q06830 Human SwissProt: P35700 Mouse Unigene: 180909 Human Unigene: 30929 Mouse Unigene: 2845 Rat 该蛋白定位于细胞质。 |
| 产品图片 |
Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human A431 cell lysates
Lane 4: Human Hela cell lysates
Primary: Anti-Peroxiredoxin 1 (bs-3875R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 23 kD
Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRDX1 Polyclonal Antibody, Unconjugated(bs-3875R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Peroxiredoxin 1 antibody (bs-3875R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
