产品编号 | bs-4008R |
英文名称 | Rabbit Anti-ATG4C antibody |
中文名称 | 自噬相关蛋白4C抗体 |
别 名 | APG4 autophagy 4 homolog C (S. cerevisiae); APG4 autophagy 4 homolog C; APG4 C; APG4-C; APG4C; ATG 4C; ATG4 autophagy related 4 homolog C (S. cerevisiae); Atg4c; ATG4C_HUMAN; AUT (S. cerevisiae) like 1, cysteine endopeptidase; AUT like 1, cysteine endopeptidase (S. cerevisiae); AUT like 1, cysteine endopeptidase; AUT like 3 cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; AUTL1; AUTL3; Autophagin 3; Autophagin-3; Autophagy related cysteine endopeptidase 3; Autophagy related protein 4 homolog C; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; Cysteine protease ATG4C; EC 3.4.22; FLJ14867; OTTHUMP00000010715. |
研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 细胞凋亡 激酶和磷酸酶 线粒体 泛素 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 52kDa |
细胞定位 | 细胞核 细胞浆 线粒体 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ATG4C: 201-300/458 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Function: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Subcellular Location: Cytoplasm (Probable). Tissue Specificity: Highly expressed in skeletal muscle, heart, liver and testis. Similarity: Belongs to the peptidase C54 family. SWISS: Q96DT6 Gene ID: 84938 Database links: Entrez Gene: 84938 Human Entrez Gene: 242557 Mouse Omim: 611339 Human SwissProt: Q96DT6 Human SwissProt: Q811C2 Mouse Unigene: 7353 Human Unigene: 241663 Mouse Unigene: 23378 Rat |
产品图片 |
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Muscle tissue lysates
Primary: Anti-ATG4C (bs-4008R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-ATG4C (bs-4008R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 55 kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |