产品中心-北京博奥森生物技术有限公司

产品编号	bs-4005R
英文名称	ATG5/APG5L
中文名称	自噬蛋白5/细胞凋亡的特异性蛋白抗体
别名	ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN.
	Specific References (15) \| bs-4005R has been referenced in 15 publications. [IF=13.281] Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 20 WB,IF ; Human; Mouse. PubMed:34672076 [IF=10.588] Jianzhao Liao. et al. Mitochondrial miR-1285 regulates copper-induced mitochondrial dysfunction and mitophagy by impairing IDH2 in pig jejunal epithelial cells. J Hazard Mater. 2022 Jan;422:126899 WB ; Pig. PubMed:34418838 [IF=9.988] Yue Zhang. et al. Endoplasmic reticulum stress-controlled autophagic pathway promotes polystyrene microplastics-induced myocardial dysplasia in birds. ENVIRON POLLUT. 2022 Oct;311:119963 WB ; Chicken. PubMed:35973452 [IF=5.64] Chengcheng Zhang. et al. Autophagy Induced by the N-Terminus of the Classic Swine Fever Virus Nonstructural Protein 5A Protein Promotes Viral Replication. Front Microbiol. 2021; 12: 733385 WB ; Pig. PubMed:34512612 [IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801 WB ; Human. PubMed:25596736 [IF=4.939] Tong-Fei Li. et al. Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma. Mol Pharmaceut. 2021;XXXX(XXX):XXX-XXX WB ; Pig. PubMed:34388342 [IF=4.622] Xiaoqi Chen. et al. Sea perch (Lateolabrax japonicus) autophagy related gene 5 promotes RGNNV infection via inhibiting RLRs-interferon signaling pathway. FISH SHELLFISH IMMUN. 2022 Aug;127:910 WB ; Fish. PubMed:35863535 [IF=4.081] Zhang, Kai. et al. Long-Term Copper Exposure Induced Excessive Autophagy of the Porcine Spleen. BIOL TRACE ELEM RES. 2022 Jul;:1-9 WB ; Pig. PubMed:35794302 [IF=3.85] Li, Yue, et al. "Autophagy contributes to 4-Amino-2-Trifluoromethyl-Phenyl Retinate-induced differentiation in human acute promyelocytic leukemia NB4 cells." Toxicology and Applied Pharmacology (2017). WB ; Human. PubMed:28130038 [IF=3.53] Lang, Fangfang, et al. "Apoptotic Cell Death Induced by Resveratrol Is Partially Mediated by the Autophagy Pathway in Human Ovarian Cancer Cells." PLOS ONE 10.6 (2015): e0129196. WB ; Human. PubMed:26067645 [IF=3.216] Lijuan Gao # et al. Chlorogenic Acid Alleviates Aβ25-35-Induced Autophagy and Cognitive Impairment via the mTOR/TFEB Signaling Pathway. Drug Des Devel Ther. 2020 May 4;14:1705-1716. WB ; Human. PubMed:32440096 [IF=3.115] Pengfei Zhang. et al. The Effects of Ginkgo biloba Extract on Autophagy in Human Macrophages Stimulated by Cigarette Smoke Extract. FRONT BIOSCI-LANDMRK. 2023 Mar;28(3):50 WB ; Human. PubMed:37005757 [IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. WB ; Mouse. PubMed:31491480 [IF=2.66] Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013). WB ; PubMed:24220541 [IF=2.33] Tang, Qishan, et al. "Resveratrol-induced apoptosis is enhanced by inhibition of autophagy in esophageal squamous cell carcinoma." Cancer letters 336.2 (2013): 325-337. WB ; Human. PubMed:23541682
研究领域	肿瘤细胞生物免疫学细胞凋亡细胞自噬
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	32kDa
细胞定位	细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human APG5L: 101-200/275
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy. Function: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Subunit: The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate. Subcellular Location: Cytoplasm. Note=Colocalizes with nonmuscle actin. Tissue Specificity: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Post-translational modifications: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. Similarity: Belongs to the ATG5 family. SWISS: Q9H1Y0 Gene ID: 9474 Database links: Entrez Gene: 9474 Human Entrez Gene: 11793 Mouse Entrez Gene: 365601 Rat Omim: 604261 Human SwissProt: Q9H1Y0 Human SwissProt: Q99J83 Mouse SwissProt: Q3MQ06 Rat Unigene: 486063 Human Unigene: 22264 Mouse Unigene: 98385 Rat
产品图片	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Kidney tissue lysates Lane 3: Rat Cerebrum tissue lysates Primary: Anti-ATG5/APG5 (bs-4005R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 47 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Blank control: A431. Primary Antibody (green line): Rabbit Anti-ATG5 antibody (bs-4005R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记