产品中心-北京博奥森生物技术有限公司

产品编号	bs-2399R
英文名称	CYP7A1 Rabbit pAb
中文名称	细胞色素P450 7A1抗体/胆固醇7a羟化酶抗体
别名	Cholesterol 7 alpha hydroxylase; Cholesterol 7 alpha monooxygenase; cholesterol 7 alpha-monooxygenase; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; CP7A; CYP 7; CYP7; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; Cytochrome P450, subfamily VIIA(cholesterol 7 alpha monooxygenase); CP7A1_HUMAN.
	Specific References (19) \| bs-2399R has been referenced in 19 publications. [IF=7.943] Shen X et al. Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice. Environ Int. 2019 Jul;128:146-157. WB ; Mouse. PubMed:31055201 [IF=5.5] Zhong D et al. Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.(2018)Cell Physiol Biochem.49(3):1163-1179. IHC&WB ; Mouse. PubMed:30196282 [IF=4.546] Li Mei. et al. Therapeutic mechanisms of the medicine and food homology formula Xiao-Ke-Yin on glucolipid metabolic dysfunction revealed by transcriptomics, metabolomics and microbiomics in mice. CHIN MED-UK. 2023 Dec;18(1):1-24 IHC ; Mouse. PubMed:37202792 [IF=4.292] Shinkichi Kosaka. et al. Bird’s eye view analysis of in situ cholesterol metabolic pathways in breast cancer patients and its clinicopathological significance in their subtypes. J Steroid Biochem. 2022 Jul;221:106103 IHC ; Human. PubMed:35367370 [IF=4.12] Ma et al. Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats. (2017) Sci.Rep. 7:44819 WB ; Rat. PubMed:28300221 [IF=3.97] Ding, Lin, et al. "Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism." Journal of Functional Foods 32 (2017): 90-97. WB ; Mouse. PubMed:doi:10.1016/j.jff.2017.02.020 [IF=3.85] Guo, Hong-li, et al. "Pyrazinamide induced rat cholestatic liver injury through inhibition of FXR regulatory effect on bile acid synthesis and transport."Toxicological Sciences (2016): kfw098. WB ; Rat. PubMed:27255380 [IF=3.743] Wang N et al. Metabolite profiling of traditional Chinese medicine XIAOPI formula: An integrated strategy based on UPLC-Q-Orbitrap MS combined with network pharmacology analysis. Biomed Pharmacother. 2020 Jan;121:109569. WB&IHF-F ; Human&rat. PubMed:31739163 [IF=3.241] Zhang L et al. Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice. Food & Function. 2019 WB ; Mouse. PubMed:31246210 [IF=3.197] Ding L et al. EPA-enriched ethanolamine plasmalogen alleviates atherosclerosis via mediating bile acids metabolism. Journal of Functional Foods,(2020) 66, 103824. WB ; Mouse. PubMed:doi:10.1016/j.jff.2020.103824 [IF=3.167] Zhenya Zhai. et al. Policosanol alleviates hepatic lipid accumulation by regulating bile acids metabolism in C57BL6/mice through AMPK–FXR–TGR5 cross-talk. 2021 Nov 03 IF ; Mouse. PubMed:34730235 [IF=2.97] Wu, Hao, et al. "The antihypercholesterolemic effect of jatrorrhizine isolated from Rhizoma Coptidis." Phytomedicine (2014). WB ; PubMed:24894270 [IF=2.96] Gabbi, Chiara, et al. "Effects of bile duct ligation and cholic acid treatment on fatty liver in two rat models of non-alcoholic fatty liver disease." Digestive and Liver Disease 44.12 (2012): 1018-1026. IHC-P ; Rat. PubMed:22883218 [IF=2.86] Nukaya, Daiki, et al. "Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas." Genes to Cells(2015). Mouse. PubMed:25727848 [IF=2.7] Kou, Shuming, et al. "Synergetic cholesterol-lowering effects of main alkaloids from Rhizoma Coptidis in HepG2 cells and hypercholesterolemia hamsters." Life Sciences (2016). WB ; Human. PubMed:26876917 [IF=2.19] Zhang, Jin-ming, et al. "Nrf2 Is Crucial For The Down-Regulation Of Cyp7a1 Induced By Arachidonic Acid In Hepg2 Cells." Environmental Toxicology and Pharmacology (2017). WB ; Human. PubMed:28364638 [IF=2.056] Yu Z et al. Lipidomic Profiling Reveals the Effect of Egg Components on non‐alcoholic Steatosis in HepG2 Cells and its Involved Mechanisms. European Journal of Lipid Science and Technology,2020, 1900451. WB ; Human. PubMed:doi:10.1002/ejlt.201900451 [IF=2.02] Wang, Shuling, et al. "Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats." Lipids in Health and Disease 13.1 (2014): 77. WB ; Rat. PubMed:24885743 [IF=1.99] Nukaya et al. Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas. (2015) Genes.Cells. 20:367-81 IF(ICC) ; Mouse. PubMed:25727848
研究领域	肿瘤细胞生物生长因子和激素转录调节因子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,GuineaPig (predicted: Mouse,Rat,Rabbit,Pig)
产品应用	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	55 kDa
检测分子量
细胞定位	细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human CYP7A1: 351-400/504
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]. Function: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Tissue Specificity: Detected in liver. Similarity: Belongs to the cytochrome P450 family. SWISS: P22680 Gene ID: 1581 Database links: Entrez Gene: 1581 Human Entrez Gene: 13122 Mouse Entrez Gene: 25428 Rat Omim: 118455 Human SwissProt: P22680 Human SwissProt: Q64505 Mouse SwissProt: P18125 Rat Unigene: 1644 Human Unigene: 57029 Mouse Unigene: 10737 Rat
产品图片	Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CYP7A1 Polyclonal Antibody, Unconjugated (bs-2399R) at 1:200 overnight at 4°C, followed by conjugat Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CYP7A1 Polyclonal Antibody, Unconjugated (bs-2399R) at 1:200 overnight at 4°C, followed by co

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记