| 产品编号 | bs-3855R |
| 英文名称 | HSD17B1 Rabbit pAb |
| 中文名称 | 羟类固醇脱氢酶17β-HSD抗体 |
| 别 名 | 11beta-HSD1; 17beta-HSD1; 17 beta HSD 1; 17 beta hydroxysteroid dehydrogenase type 1; 17β-hydroxysteroid dehydrogenasetype 1 EDH17B2; EDHB17; Estradiol 17 beta dehydrogenase 1; HSD17; Hydroxysteroid(17 beta) dehydrogenase 1; Placental 17 beta hydroxysteroid dehydrogenase; DHB1_HUMAN. |
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Specific References (1) | bs-3855R has been referenced in 1 publications.
[IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017). IHC-P ; Mouse.
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| 研究领域 | 肿瘤 免疫学 生长因子和激素 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSD17B1: 21-120/328 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] Subunit: Homodimer. Subcellular Location: Cytoplasm. Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. SWISS: P14061 Gene ID: 3292 Database links: Entrez Gene: 3292 Human Omim: 109684 Human SwissProt: P14061 Human Unigene: 654385 Human Unigene: 655222 Human Ⅰ型17β-羟类固醇脱氢酶又称Ⅰ型17HSD，催化雌酮与雌二醇之间的转化，为性激素合成过程中最后步骤的催化酶。它催化性激素Ｃ17位上的酮基和醇基之间的还原和氧化反应 ,使低生物活性的雌酮、雄烯二酮与高生物活性的雌二醇、睾酮之间相互转化。17βHSD的结构和功能的异常与一些疾病 ,如肿瘤、假两性等的发生有密切关系. |
| 产品图片 |
Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti- HSD17B1 (bs-3855R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
