| 产品编号 | bs-3858R |
| 英文名称 | Rabbit Anti-SH2D2A antibody |
| 中文名称 | 血管内皮生长因子受体相关蛋白抗体 |
| 别 名 | SH2D2A/VEGF receptor associated protein SCAP; SH2 domain containing adapter protein; SH2 domain containing protein 2A; SH2 domain protein 2A; T cell specific adapter protein; T cell specific adapter protein TSAd; T cell specific adpater protein TSAd; T lymphocyte specific adaptor protein; TSAd; VEGF receptor associated protein; VRAP. |
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Specific References (1) | bs-3858R has been referenced in 1 publications.
[IF=12.35] Compeer et al. A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation. (2018) Nat.Commun. 9:1597 IF ; Mouse.
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| 研究领域 | 肿瘤 免疫学 生长因子和激素 血管内皮细胞 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SH2D2A: 101-200/389 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SH2D2A is thought to be a T-cell-specific adapter protein involved in the control of T-cell activation. It may play a role in the CD4-p56-LCK-dependent signal transduction pathway and also in normal and pathological angiogenesis. Subunit: Interacts with KDR. Interacts with TXK and ITK. Subcellular Location: Cytoplasmic Tissue Specificity: SH2D2A is thought to be a T-cell-specific adapter protein involved in the control of T-cell activation. It may play a role in the CD4-p56-LCK-dependent signal transduction pathway and also in normal and pathological angiogenesis. Similarity: Contains 1 SH2 domain. SWISS: Q9NP31 Gene ID: 9047 Database links: Entrez Gene: 9047 Human Entrez Gene: 27371 Mouse Omim: 604514 Human SwissProt: Q9NP31 Human SwissProt: Q9QXK9 Mouse Unigene: 103527 Human Unigene: 86361 Mouse |
| 产品图片 |
Blank control(blue): HUVEC cells(fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- SH2D2A antibody(bs-3858R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
