| 产品编号 | bs-3857R |
| 英文名称 | Steroid sulfatase Rabbit pAb |
| 中文名称 | 类固醇硫酸酯酶抗体 |
| 别 名 | ARSC; ARSC1; Arylsulfatase C; Arylsulfatase C isozyme S; ASC; EC 3.1.6.2; ES; Estrone sulfatase; SSDD; Steroid sulfatase(microsomal); Steroid sulfatase(microsomal) arylsulfatase C isozyme S; Steroid sulfatase; Steryl sulfatase; Steryl sulfatase precursor; Steryl sulfate sulfohydrolase; STS; STS_HUMAN; Steryl-sulfatase; ASC; Steryl-sulfate sulfohydrolase. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Steroid sulfatase: 51-150/583 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI). [provided by RefSeq, Jul 2008]. Function: Conversion of sulfated steroid precursors to estrogens during pregnancy. Subunit: Homodimer. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Post-translational modifications: The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. DISEASE: Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks. Similarity: Belongs to the sulfatase family. SWISS: P08842 Gene ID: 412 Database links: Entrez Gene: 412 Human Omim: 308100 Human SwissProt: P08842 Human Unigene: 522578 Human Unigene: 700558 Human Unigene: 700559 Human 类固醇硫酸酯酶SSDD缺乏，可导致病人的皮肤培养的纤维母细咆、滋养层细胞、外周白细胞、毛球的角化组织、表皮细胞、角质层和甲等变化，见于X连锁鱼鳞病(XLI)。 |
| 产品图片 |
Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- Steroid sulfatase (bs-3857R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 63 kD
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human HepG2 cell lysates
Primary: Anti-Steroid sulfatase (bs-3857R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
