| 产品编号 | bs-3809R |
| 英文名称 | thymidine phosphorylase Rabbit pAb |
| 中文名称 | 胸苷磷酸化酶抗体 |
| 别 名 | ECGF 1; PDEGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Gliostatin; hPD ECGF; MNGIE; PD ECGF; PDECGF; PDEGF; Platelet derived endothelial growth factor; TdRPase; TP; TYMP; Thymidine phosphorylase; TYPH_HUMAN; TdRPase. |
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Specific References (1) | bs-3809R has been referenced in 1 publications.
[IF=7.514] Yi Wu. et al. Selective transportation and energy homeostasis regulation of dietary advanced glycation end-products in human intestinal Caco-2 cells. FOOD CHEM. 2022 Oct;391:133284 IF,FC ; Human.
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| 研究领域 | 肿瘤 细胞生物 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Thymidine Phosphorylase: 121-220/482 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In the presence of inorganic orthophosphate, the platelet-derived endothelial growth factor (PD-ECGF) thymidine phosphorylase (TP) (gliostatin) catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites. Function: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Subunit: Homodimer. DISEASE: Defects in TYMP are the cause of mitochondrial DNA depletion syndrome type 1 (MTDPS1) [MIM:603041]; also known as myoneurogastrointestinal encephalomyopathy. A multisystem disease associated with mitochondrial dysfunction. It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, thin body habitus, peripheral neuropathy, and myopathy. Similarity: Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. SWISS: P19971 Gene ID: 1890 Database links: Entrez Gene: 1890 Human Entrez Gene: 72962 Mouse Omim: 131222 Human SwissProt: P19971 Human SwissProt: Q99N42 Mouse Unigene: 180903 Human Unigene: 730607 Human Unigene: 287977 Mouse Unigene: 219088 Rat 胸苷磷酸化酶（TP）是一种分子量为50-55kDa的蛋白，在氟嘧啶转化过程中起重要作用。TP具有血管生成活性，与血小板源性内皮细胞生长因子的结构和作用相似，因此又被称为肿瘤相关血管生长因子。其可能通过血管生成作用促进癌细胞的生长和转移。 |
| 产品图片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-thymidine phosphorylase (bs-3809R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated (bs-3809R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TYPH) Polyclonal Antibody, Unconjugated (bs-3809R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
