| 产品编号 | bs-3699R |
| 英文名称 | Rabbit Anti-GPR15 antibody |
| 中文名称 | G蛋白偶联受体15抗体 |
| 别 名 | GPCR GPR15; BOB; G protein coupled receptor 15; G protein coupled receptor GPR15; GPR15_HUMAN; G-protein coupled receptor 15; Brother of Bonzo. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 G蛋白偶联受体 细胞表面分子 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR15: 51-150/360 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012] Function: Probable chemokine receptor. Alternative coreceptor with CD4 for HIV-1 infection. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49685 Gene ID: 2838 Database links: Entrez Gene: 2838 Human Entrez Gene: 71223 Mouse Omim: 601166 Human SwissProt: P49685 Human SwissProt: Q0VDU3 Mouse Unigene: 563128 Human Unigene: 359970 Mouse Unigene: 426544 Mouse G蛋白偶联受体是一种细胞表面受体，能将细胞外的分子信号传递至细胞内激起细胞反应，对多种细胞行起调控作用。G蛋白偶联受体（GPCR）参与了机体内多种生理过程的调节，是目前治疗多种人类疾病包括代谢性疾病的药物靶点。 |
| 产品图片 |
Tissue/cell: Mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR15 Polyclonal Antibody, Unconjugated(bs-3699R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat stomach tissue; Frozen section;
Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR15 Polyclonal Antibody, Unconjugated(bs-3699R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
