| 产品编号 | bs-3675R |
| 英文名称 | R-cadherin |
| 中文名称 | R-钙粘附分子抗体 |
| 别 名 | Cadherin 4; Cadherin4; Cadherin-4 CDH4; R CAD; Retinal Cadherin. |
| 研究领域 | 肿瘤 细胞生物 免疫学 激酶和磷酸酶 细胞粘附分子 糖蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Chicken,Dog,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 81kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human R-cadherin: 401-500/916 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cadherins constitute a superfamily of calcium-dependent intercellular adhesion molecules and are thought to play a role in cell recognition and segregation, morphogenetic regulation, and tumor suppression. R Cadherin is expressed mainly in the brain but is also found in other tissues. Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed mainly in brain but also found in other tissues. Similarity: Contains 5 cadherin domains. SWISS: P55283 Gene ID: 1002 Database links: Entrez Gene: 1002 Human Entrez Gene: 12561 Mouse Omim: 603006 Human SwissProt: P55283 Human SwissProt: P39038 Mouse SwissProt: Q80ZV4 Mouse Unigene: 473231 Human Unigene: 598638 Human Unigene: 184711 Mouse Unigene: 382094 Mouse Unigene: 392073 Mouse Unigene: 214089 Rat R-钙粘附分子,是依赖钙离子的细胞间糖蛋白粘附分子的一种，主要是参与细胞间同源性粘附、调控和介导细胞间相互反应，在肿瘤的迁移、分化中起到一定的作用。在脑组织中表达外，其他组织中已有定位。 |
| 产品图片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-R-cadherin/Cadherin 4 Polyclonal Antibody, Unconjugated(bs-3675R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
