| 产品编号 | bs-3674R |
| 英文名称 | Rabbit Anti-TSPO/PBR antibody |
| 中文名称 | 外周苯二氮受体抗体 |
| 别 名 | BPBS; BZRP; DBI; IBP; Isoquinoline carboxamide-binding protein; MBR; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBS; Peripheral benzodiazepine receptor; Peripheral benzodiazepine receptor-related protein; Peripheral type benzodiazepine receptor; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; translocator protein (18kDa); Translocator protein; TSPO; Tspo1; Benzodiazapine receptor (peripheral); Benzodiazepine peripheral binding site. |
| 研究领域 | 细胞生物 免疫学 神经生物学 转录调节因子 糖尿病 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 18kDa |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PBR: 31-130/169 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PBR (Peripheral type Benzodiazepine Receptor) is a ubiquitous protein (18 kDa) found in most steroidogenic tissues. It is located primarily in the outer mitochondrial membrane of steroid producing tissues and is found in association with a 34 kDa voltage dependent anion channel protein (VDAC). PBR interacts with some benzodiazepines and has different affinities than its endogenous counterpart. PBR appears to be a key factor in the transport of cholesterol from the outer to the inner mitochondrial membrane to permit the initiation of steroid hormone synthesis. It is thought to be part of the mitochondrial permeability transition pore (PT) and to be involved in apoptosis. It is speculated that patients with congenital lipoid adrenal hyperplasia, who cannot make any steroids, might have a genetic lesion in BZRP. A short form, PBRS is also expressed in the same tissues, but at a level about ten times that of PBR. Function: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Subcellular Location: Mitochondrion membrane. Tissue Specificity: Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. Similarity: Belongs to the TspO/BZRP family. SWISS: B1AH88 Gene ID: 706 Database links: Entrez Gene: 706 Human Entrez Gene: 12257 Mouse Omim: 109610 Human SwissProt: B1AH88 Human SwissProt: P30536 Human SwissProt: P50637 Mouse Unigene: 202 Human Unigene: 1508 Mouse 地西泮结合抑制因子； DBI能拮抗安定物质：diazepam与脑突触膜相结合 ,故称之为安定结合抑制蛋白(DBI)，地西泮结合抑制因子(diazepam binding inhibitor,DBI)具有抑制由葡萄糖诱导的胰岛素分泌、促进胆固醇跨线粒体膜转运和调节脂肪酸合成与代谢等多种生理功能。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBR) Polyclonal Antibody, Unconjugated (bs-3674R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PBR) Polyclonal Antibody, Unconjugated (bs-3674R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSPO/PBR) Polyclonal Antibody, Unconjugated (bs-3674R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-TSPO/PBR antibody (bs-3674R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
