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Rabbit Anti-KEAP1 antibody (bs-3648R)

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说明书： 50ul 100ul 200ul

50ul/1180.00元

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100ul/1980.00元

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产品编号	bs-3648R
英文名称	Rabbit Anti-KEAP1 antibody
中文名称	胞质接头蛋白Keap1抗体
别名	Cytosolic inhibitor of Nrf2; INrf 2; INrf2; Keap 1; KEAP1; KEAP1_HUMAN; Kelch like ECH associated protein 1; Kelch like protein 19; Kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132; KLHL 19; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454.
	Specific References (26) \| bs-3648R has been referenced in 26 publications. [IF=7.675] Xiaoxiao Zou. et al. Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice. ANTIOXIDANTS-BASEL. 2023 Mar;12(3):712 WB ; Mouse. PubMed:36978962 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965 WB ; Duck. PubMed:34775344 [IF=5.81] Xinyue Chang. et al. The Protective Effect of Trichilia catigua A. Juss. on DEHP-Induced Reproductive System Damage in Male Mice. Front Pharmacol. 2022; 13: 832789 IHC ; Mouse. PubMed:35185586 [IF=5.552] Huang, Jianjia. et al. Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways. J ZHEJIANG UNIV-SC B. 2023 Feb;24(2):157-171 WB ; Dog. PubMed:36751701 [IF=5.195] Yiyun Qian. et al. Seeds of Ginkgo biloba L. inhibit oxidative stress and inflammation induced by cigarette smoke in COPD rats through the Nrf2 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115758 WB ; Rat. PubMed:36167232 [IF=4.868] Chen W et al. Obeticholic Acid Protects against Gestational Cholestasis-Induced Fetal Intrauterine Growth Restriction in Mice. Oxidative Medicine and Cellular Longevity, 2019, 1–17. WB ; Mouse. PubMed:doi:10.1155/2019/7419249 [IF=4.545] Yingran Liang. et al. Crocin ameliorates arsenic trioxide‑induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713 WB ; Rat. PubMed:32920515 [IF=4.529] Shikuo Rong. et al. Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-κB and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia. MEDIAT INFLAMM. 2022 Dec 06;2022:5184721 WB ; Mouse. PubMed:36523959 [IF=4.389] Junrong Zhang. et al. Neuroprotective effects of maize tetrapeptide-anchored gold nanoparticles in Alzheimer’s disease. Colloid Surface B. 2021 Apr;200:111584 WB ; Mouse. PubMed:33508658 [IF=4.36] Song Yang. et al. Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo. J Ethnopharmacol. 2021 Oct;:114739 WB ; Mouse. PubMed:34648903 [IF=4.19] Su, Chuanyang, et al. "Tetrachlorobenzoquinone activates Nrf2 signaling by Keap1 cross-linking and ubiquitin translocation, but not Keap1-Cullin3 complex dissociation." Chemical Research in Toxicology (2015). WB ; Human. PubMed:25742418 [IF=4.109] Yingrong Ye. et al. Investigations into ferroptosis in methylmercury-induced acute kidney injury in mice. ENVIRON TOXICOL. 2023 Mar;: WB ; Mouse. PubMed:36880449 [IF=4.105] Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1α proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963 WB ; Human. PubMed:33444648 [IF=3.905] Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025 IF ; Human. PubMed:35026282 [IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653 WB ; Human. PubMed:28974930 [IF=3.743] Jin W et al. Tannic acid ameliorates arsenic trioxide-induced nephrotoxicity, contribution of NF-κB and Nrf2 pathways. Biomed Pharmacother. 2020 Jun;126:110047. WB ; rat. PubMed:32146384 [IF=3.639] Huiling Mao. et al. Leucine protects bovine intestinal epithelial cells from hydrogen peroxide-induced apoptosis by alleviating oxidative damage. J SCI FOOD AGR. 2022 May 05 WB ; Bovine. PubMed:35437753 [IF=3.58] Su, Chuanyang, et al. "The electrophilic character of quinones is essential for the suppression of Bach1." Toxicology (2017). PubMed:28645578 [IF=3.571] Zhao Y et al. Lycopene prevents DEHP-induced Leydig cell damage with the Nrf2 antioxidant signaling pathway in mice. J Agric Food Chem. 2019 Dec 8. WB ; Mouse. PubMed:31814398 [IF=3.23] Chen, Ya-Hui, et al. "Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia." PLOS ONE11.4 (2016): e0154210. WB, IHC-P ; Rabbit. PubMed:27124120 [IF=3.205] Meng et al. Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1. (2018) J.Anim.Sci.Biotechnol. 9:34 WB ; Sow. PubMed:29713468 [IF=3.11] Su, Chuanyang, et al. "Neohesperidin dihydrochalcone against CCl4-induced hepatic injury through different mechanisms The implication of free radical scavenging and Nrf2 activation." Journal of Agricultural and Food Chemistry(2015). WB ; Human. PubMed:25978654 [IF=2.639] Jin Houet al. Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway. Biol Trace Elem Res . 2021 Mar;199(3):1062-1073. WB ; mouse. PubMed:32666434 [IF=1.892] Kaiqiang Fu. et al. Tanshinone IIA exhibits anti-inflammatory and antioxidative effects in LPS-stimulated bovine endometrial epithelial cells by activating the Nrf2 signaling pathway. Res Vet Sci. 2021 May;136:220 WB ; Bovine. PubMed:33689878 [IF=1.719] Kacar S et al. Effect of acrylamide on BEAS-2B normal human lung cells: Cytotoxic, oxidative, apoptotic and morphometric analysis. Acta Histochem. 2019 Jul;121(5):595-603. ICC ; Human. PubMed:31109687 [IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27 WB ; Bovine. PubMed:33866621
研究领域	肿瘤细胞生物免疫学信号转导细胞凋亡转录调节因子激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog)
产品应用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	69kDa
细胞定位	细胞核细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human KEAP1: 65-160/624
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. Subunit: Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Subcellular Location: Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus. Tissue Specificity: Broadly expressed, with highest levels in skeletal muscle. Similarity: Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats. SWISS: Q14145 Gene ID: 9817 Database links: Entrez Gene: 9817 Human Entrez Gene: 50868 Mouse Entrez Gene: 100136900 Pig Entrez Gene: 117519 Rat Omim: 606016 Human SwissProt: Q14145 Human SwissProt: Q9Z2X8 Mouse SwissProt: Q684M4 Pig SwissProt: P57790 Rat Unigene: 465870 Human Unigene: 248266 Mouse Unigene: 23467 Rat Keap1是细胞抗氧化反应的重要调节蛋白，Keap1与Nrf2/ARE构成的信号通路在抗肿瘤、抗应激、调节GSH合成、抗凋亡、抗炎反应、神经保护等方面发挥着广泛的细胞保护作用。
产品图片	This image was generously provided by Yang Song, Ph.D. at Southwest University in Chong Qing, China. HepG2 cells were incubated with Rabbit Anti-KEAP1 Polyclonal Antibody (bs-3648R) at 4°C overnight and then mixed with Protein A agarose beads at 4°C for 3hrs. The solutions were centrifuged and the pellets were washed with lysis buffer, heated, and subsequently analyzed by Western blotting. Sample: A673(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-KEAP1 (bs-3648R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (bs-3648R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	AbBy Fluor 350 标记
	AbBy Fluor 488 标记
	AbBy Fluor 555 标记
	AbBy Fluor 594 标记
	AbBy Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记