| 产品编号 | bs-3643R |
| 英文名称 | PLASTIN3 Rabbit pAb |
| 中文名称 | 丝束蛋白T Plastin抗体 |
| 别 名 | BMND18; DIH5; T-plastin; PLST_HUMAN; PLS3; T-fimbrin; PLST_MOUSE; PLST_RAT; |
| 研究领域 | 细胞生物 免疫学 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Plastin-3: 401-500/630 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
T Plastin is a representative of an important family of actin-cross-linking and -bundling proteins that organize most of the actin networks in cells. T Plastin is associated with the actin core bundle of microvilli, stereocilia and microspikes in epithelial cells and was proposed to participate in the assembly of these structures. In mesenchymal cells, T plastin localizes to focal adhesions and the leading edge, a broad lamellar membrane extension harboring a dynamic branched actin network. Recent studies have shown that T plastin has the potential to be a Sezary cell-specific marker valuable for diagnostic and treatment of Sezary syndrome. Function: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in a variety of organs, including muscle, brain, uterus and esophagus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR Similarity: Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains. SWISS: P13797 Gene ID: 5358 Database links: Entrez Gene: 5358 Human Entrez Gene: 102866 Mouse Omim: 300131 Human SwissProt: P13797 Human SwissProt: Q99K51 Mouse Unigene: 496622 Human Unigene: 28777 Mouse Unigene: 32103 Rat 丝束(纤毛素)是肌动蛋白结合蛋白家族的成员,存在于细胞支架的重组中. 丝束3是来源于纤毛素蛋白家族的T淋巴细胞特异肌动蛋白成束蛋白。近年来T Plastin在用于sezary cell综合征的研究方面较多。 |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-PLASTIN3 (bs-3643R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLASTIN3) Polyclonal Antibody, Unconjugated (bs-3643R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PLASTIN3 antibody (bs-3643R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
