| 产品编号 | bs-3597R |
| 英文名称 | DDX4 Rabbit pAb |
| 中文名称 | DDX4抗体 |
| 别 名 | DDX 4; DEAD(Asp Glu Ala Asp) box polypeptide 4; Dead box protein 4; Dead-box protein 4; MGC111074; MVH; Probable ATP dependent RNA helicase DDX4; VASA; VASA homolog; DDX4_HUMAN.; DDX4; |
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Specific References (6) | bs-3597R has been referenced in 6 publications.
[IF=14.224] Wenqing Xu. et al. Oral exposure to polystyrene nanoplastics reduced male fertility and even caused male infertility by inducing testicular and sperm toxicities in mice. J HAZARD MATER. 2023 Jul;454:131470 IF ; Mouse.
[IF=4.996] Klein, Sabine. et al. Identification of chicken LOC420478 as Bucky ball equivalent and potential germ plasm organizer in birds. SCI REP-UK. 2022 Oct;12(1):1-15 IHC ; Chicken.
[IF=3.471] Nakami Wilkister Nabulindo. et al. Culture of Kenyan Goat (Capra hircus) Undifferentiated Spermatogonia in Feeder-Free Conditions. FRONT VET SCI. 2022; 9: 894075 ICC ; Goat.
[IF=3.195] Xia, Yuguo. et al. Gut microbiota involved in spermatogenic function of Sancai Lianmei granules in obese mice. N-S ARCH PHARMACOL. 2022 Sep;:1-15 IF ; Mouse.
[IF=1.786] Qin X et al. Efficient knock-in at the chicken ovalbumin locus using adenovirus as a CRISPR/Cas9 delivery system. 3 Biotech,2019, 9(12). FCM ; Chicken.
[IF=1.447] Tang X et al. Retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) restore the germline competence of in vitro cultured chicken blastodermal cells. In Vitro Cell Dev Biol Anim. 2019 Mar;55(3):169-176. IF,WB&FCM ; Chicken.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 80 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DDX4/MVH: 601-700/724 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Function: May play a role in germ cell development. May play a role in sperm motility. Subunit: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Subcellular Location: Cytoplasm. Cytoplasm, perinuclear region. Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Similarity: Belongs to the DEAD box helicase family. DDX4/VASA subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Database links: Entrez Gene: 54514 Human Entrez Gene: 13206 Mouse Omim: 605281 Human SwissProt: Q9NQI0 Human SwissProt: Q61496 Mouse Unigene: 223581 Human Unigene: 12818 Mouse Unigene: 198577 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Primary: Anti-DDX4 (bs-3597R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 76 kDa
Sample:
Lane 1: Testis (Mouse) Tissue Lysate at 40 ug
Primary: Anti- DDX4 (bs-3597R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 78 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
