| 产品编号 | bs-3422R |
| 英文名称 | Phospho-TCTP (Ser46) Rabbit pAb |
| 中文名称 | 磷酸化翻译控制肿瘤蛋白抗体 |
| 别 名 | TCTP(phospho Ser46); TCTP(phospho S46); Fortilin; Histamine-releasing factor; p02; p23; TPT1; translationally controlled tumor protein; tumor protein translationally-controlled 1; TCTP_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Cow,Dog,GuineaPig,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human TCTP around the phosphorylation site of Ser46: DD(p-S)LI |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM). Function: Involved in calcium binding and microtubule stabilization. Subunit: Interacts with STEAP3. Subcellular Location: Cytoplasm. Tissue Specificity: Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate. Similarity: Belongs to the TCTP family. SWISS: P13693 Gene ID: 7178 Database links: Entrez Gene: 7178 Human Entrez Gene: 22070 Mouse Omim: 600763 Human SwissProt: P13693 Human SwissProt: P63028 Mouse Unigene: 374596 Human Unigene: 296922 Mouse Unigene: 297482 Mouse Unigene: 36610 Rat 翻译控制肿瘤蛋白最初发现于肿瘤组织，后来又从其他组织中相继分离出来,是许多动物肿瘤细胞中存在的一类较丰富的蛋白,TCTP参与钙离子结合和微管的稳定,是一类广泛存在于动物、植物及酵母中的、在序列上高度保守且具有很高表达量的蛋白家族。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TCTP (Ser46)) Polyclonal Antibody, Unconjugated (bs-3422R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
