| 产品编号 | bs-3341R |
| 英文名称 | Phospho-PLCB3 (Ser1105) Rabbit pAb |
| 中文名称 | 磷酸化磷酯酶Cβ3抗体 |
| 别 名 | PLCB3_HUMAN; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-3; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-3; EC:3.1.4.11; phosphoinositide phospholipase C-beta-3; phospholipase C-beta-3(PLC-beta-3); SMDCD; phospholipase C beta 3; |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 产品应用 | Flow-Cyt=1μg /Test,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 136 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human PLC beta3 around the phosphorylation site of Ser1105: N(p-S)ISE |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Phospholipase C is a key enzyme in phosphatidylinositol (PIP2) metabolism and lipid signaling pathways. The Phospholipase C family consists of 13 isozymes split between six subfamilies. Phospholipase C beta is activated by G protein subunits. Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Subunit: Interacts with SHANK2. Interacts with LPAR2. Subcellular Location: Membrane; Peripheral membrane protein. Similarity: Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. SWISS: Q01970 Gene ID: 5331 Database links: Entrez Gene: 5331 Human Entrez Gene: 18797 Mouse Omim: 600230 Human SwissProt: Human SwissProt: P51432 Mouse Unigene: 523761 Human Unigene: 591953 Human Unigene: 273204 Mouse Unigene: 16983 Rat |
| 产品图片 |
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PLC beta3 (Ser1105) ) polyclonal Antibody, Unconjugated (bs-3341R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control(blue): RAJI(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-Phospho-PLC beta3 (Ser1105) antibody(bs-3341R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
