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PFKFB3/PFK2 Rabbit pAb (bs-3528R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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产品编号 bs-3528R
英文名称 PFKFB3/PFK2 Rabbit pAb
中文名称 果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗体
别    名 PFK2; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3; 6PF-2-K/Fru-2, 6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2, 6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2; 6-phosphofructo-2-kinase; Fructose-2, 6-bisphosphatase; F263_HUMAN.  
Specific References  (1)     |     bs-3528R has been referenced in 1 publications.
[IF=3.231] Lifang Li. et al. Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome. ANIMALS. 2022 Jan;12(19):2488  WB ;  Chicken.  
研究领域 肿瘤  免疫学  信号转导  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,GuineaPig,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 60 kDa
检测分子量
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PFKFB3: 401-520/520 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]

Function:
Synthesis and degradation of fructose 2,6-bisphosphate.

Tissue Specificity:
Ubiquitous.

Similarity:
In the C-terminal section; belongs to the phosphoglycerate mutase family.

SWISS:
Q16875

Gene ID:
5209

Database links:

Entrez Gene: 5209 Human

Entrez Gene: 170768 Mouse

Entrez Gene: 24640 Rat

Omim: 605319 Human

SwissProt: Q16875 Human

SwissProt: P25114 Rat

Unigene: 444304 Human

Unigene: 44844 Rat




IPFK2是糖酵解的一种重要酶,广泛存在于各种生物细胞中。由于该酶对调控糖酵解通路的重要作用.
产品图片
Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human U251 cell lysates Lane 4: Human A431 cell lysates Lane 5: Human Jurkat cell lysates Primary: Anti-PFKFB3/PFK2 (bs-3528R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PFKFB3/PFK2) Polyclonal Antibody, Unconjugated (bs-3528R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PFKFB3/PFK2) Polyclonal Antibody, Unconjugated (bs-3528R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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