| 产品编号 | bs-2222R |
| 英文名称 | Rabbit Anti-Endoglucanase 25 antibody |
| 中文名称 | 内切葡聚糖酶25抗体 |
| 别 名 | GUN25_ARATH; Endoglucanase 25; Cellulase homolog OR16pep; Endo-1,4-beta glucanase 25; Protein KORRIGAN; Protein RADIALLY SWOLLEN 2. 内切葡聚糖酶25; 纤维素酶同源物OR16pep; |
| 研究领域 | 药物及化合物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Arabidopsis Thaliana) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana Endoglucanase 25: 101-200/621 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides. Cellulases break down the cellulose molecule into monosaccharides ("simple sugars") such as beta-glucose, or shorter polysaccharides and oligosaccharides. Cellulose breakdown is of considerable economic importance, because it makes a major constituent of plants available for consumption and use in chemical reactions. The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, and cereal beta-D-glucans. Because cellulose molecules bind strongly to each other, cellulolysis is relatively difficult compared to the breakdown of other polysaccharides such as starch. Function: Required for cellulose microfibrils formation. Involved in cell wall assembly during cell elongation and cell plate maturation in cytokinesis. Required for secondary cell wall formation in the developing xylem. May cycle through different intracellular compartments, including plasma membrane. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Note=Cell plate. Tissue Specificity: Highly expressed in roots and stems, at intermediate levels in leaves and flowers, and at lower levels in siliques. Expressed in xylem (at protein level). Post-translational modifications: Glycosylated. N-glycosylation of KOR in the endoplasmic reticulum followed by N-glycan modifications in the Golgi are essential for catalytic activity. Similarity: Belongs to the glycosyl hydrolase 9 (cellulase E) family. SWISS: Q38890 Gene ID: 835035 Database links:
纤维素酶(cellulase) 它是与脱落直接有关的酶，菜豆、棉花和柑橘叶片脱落时，纤维素酶活性增加。从菜豆叶柄离区中分离出pI9.5和pI4.5(分别称为9.5或4.5纤维素酶)两种纤维素酶，前者与细胞壁木质化有关，受IAA调控；后者与细胞壁降解有关，受乙烯调控。测定柑橘小叶片离区的各个不同区段中的纤维素酶活性，发现酶活性最高的部位是在离区的近轴端（靠近茎的0.22mm处），所以纤维素酶的活性不一定与离层细胞的分开直接有关，而可能与离层分离后的保护层发育的关系更为直接。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
