| 产品编号 | bs-3181R |
| 英文名称 | Rabbit Anti-Phospho-HSP90 alpha (Thr5 + Thr7) antibody |
| 中文名称 | 磷酸化热休克蛋白90α抗体 |
| 别 名 | HSP90 alpha (Phospho-Thr5/7); HS90A_HUMAN; heat shock 90kDa protein 1 alpha; Heat shock protein 90kDa alpha cytosolic class A member 1; heat shock protein 90kDa alpha (cytosolic), class A member 2; Heat shock protein 90kDa alpha cytosolic class B member 1; Heat shock protein HSP 90 alpha; Heat shock protein HSP 90-alpha; Heat shock protein HSP 90 beta; HSP 84; HSP 86; Hsp 90; HSP84; HSP86; Hsp89; HSP89A; Hsp90; HSP90 Beta; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSP90N; HSPC1; HSPC2; HSPCA; HSPCAL1; HSPCAL3; HSPCAL4; HSPCB; HSPN; Heat shock 86 kDa; HSP90ALPHA; HSPN; LAP2; Lipopolysaccharide associated protein2; LPS associated protein 2; NY REN 38 antigen; Renal carcinoma antigen NY REN 38; Renal carcinoma antigen NY-REN-38; D6S182; FLJ26984; FLJ31884. |
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Specific References (1) | bs-3181R has been referenced in 1 publications.
[IF=4.145] Wanqing Zhou. et al. LncRNA-MEG3 attenuates hyperglycemia-induced damage by enhancing mitochondrial translocation of HSP90A in the primary hippocampal neurons. EXP CELL RES. 2022 Aug;:113320 CoIP, WB ; Rat.
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| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 84kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human HSP90 around the phosphorylation site of Thr5 + Thr7: EE(p-T)Q(p-T)QD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways. Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Subunit: Homodimer. Interacts with AHSA1, FNIP1, HSF1, SMYD3 and TOM34. Interacts with TERT; the interaction, together with PTGES3, is required for correct assembly and stabilization of the TERT holoenzyme complex. Interacts with CHORDC1 and DNAJC7. Interacts with STUB1 and UBE2N; may couple the chaperone and ubiquitination systems. Subcellular Location: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: ISGylated. S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. Similarity: Belongs to the heat shock protein 90 family. SWISS: P07900 Gene ID: 3320 Database links: Entrez Gene: 423463 Chicken Entrez Gene: 3320 Human Entrez Gene: 15519 Mouse Omim: 140571 Human SwissProt: P11501 Chicken SwissProt: P07900 Human SwissProt: P07901 Mouse SwissProt: P30946 Rabbit Unigene: 525600 Human Unigene: 700831 Human Unigene: 1843 Mouse Unigene: 341186 Mouse Unigene: 119867 Rat 热休克蛋白-90α主要在类肌细胞、间质细胞、支持细胞核及生精细胞的胞浆表达; HSP-90 alpha与肿瘤的关系密切，在许多肿瘤细胞中HSP90α表达升高. |
| 产品图片 |
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-HSP90 alpha (Thr5+Thr7) Polyclonal Antibody, Unconjugated(bs-3181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-HSP90 alpha (Thr5 + Thr7)) Polyclonal Antibody, Unconjugated (bs-3181R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
