| 产品编号 | bs-3096R |
| 英文名称 | Rabbit Anti-Phospho-cdc25C (Ser216) antibody |
| 中文名称 | 磷酸化细胞分裂周期蛋白25C抗体 |
| 别 名 | cdc25C(Phospho-Ser216); cdc25C(Phospho Ser216); cdc25C(Phospho S216); CDC 25; Cdc 25C; CDC25; CDC25C; Cell division cycle 25 homolog C; Cell Division Cycle 25C; Cell Division Cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; M-phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; MPIP3_HUMAN; Phosphotyrosine phosphatase. |
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Specific References (1) | bs-3096R has been referenced in 1 publications.
[IF=2.976] Chen X et al. Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells.(2019)Oncol Rep. Feb;41(2):765-778. WB ;
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| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216: P(p-S)MP |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008] Function: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. Subunit: Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity. Interacts with MAPK14 and 14-3-3 proteins. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity. Similarity: Belongs to the MPI phosphatase family. Contains 1 rhodanese domain. SWISS: P30307 Gene ID: 995 Database links: Entrez Gene: 995 Human Omim: 157680 Human SwissProt: P30307 Human Unigene: 656 Human CDC25家族的组成哺乳动物中CDC25家族一共包括3种同源异构体（CDC25A、CDC25B、CDC25C），约有50%的序列同源，是一组在细胞周期调控中发挥巨大作用的苏/酪氨酸双功能酶 。不同的CDC25家族蛋白在细胞周期中的作用时相亦有差异，CDC25A和CDC25C分别在S期和M期发挥主要作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
