| 产品编号 | bs-3063R |
| 英文名称 | Phospho-CEBP beta (Thr235) |
| 中文名称 | 磷酸化转录调节因子CEBP β抗体 |
| 别 名 | P-CEBP beta (Thr235); P-CEBP beta (T235); CEBP-beta; CLEBP beta; CCAAT Enhancer Binding Protein beta; AGP/EBP; C EBP beta; C/EBP related protein 2; CEBPB; CRP2; IL 6DBP; IL6DBP; Interleukin 6 dependent binding protein; LAP; Liver enriched transcriptional activator; NF IL6; NFIL6; Nuclear factor NF IL6; SF B; SFB; Silencer factor B; TCF5; Transcription factor 5; C/EBP beta; CCAAT/enhancer-binding protein beta; CEBPB_HUMAN; Liver activator protein; Liver enriched transcriptional activator; Nuclear factor NF IL6; Nuclear factor NF-IL6; TCF-5. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Dog,Pig,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg /test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CEBP beta around the phosphorylation site of Thr235: PG(p-T)PS |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP alpha, CEBP delta, and CEBP gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL1 response element in the IL6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. Function: Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. Subunit: Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Forms a complex with THOC5 (By similarity). Interacts with ZNF638; this interaction increases transcriptional activation (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Expressed at low levels in the lung, kidney and spleen. Similarity: Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain. SWISS: P17676 Gene ID: 1051 Database links: Entrez Gene: 1051 Human Entrez Gene: 12608 Mouse Omim: 189965 Human SwissProt: P17676 Human SwissProt: P28033 Mouse Unigene: 517106 Human Unigene: 719041 Human Unigene: 720603 Human Unigene: 439656 Mouse Unigene: 6479 Rat |
| 产品图片 |
Blank control (blue line): Hela(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Phospho-CEBP beta (Thr235) antibody (bs-3063R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
