| 产品编号 | bs-2723R |
| 英文名称 | Rabbit Anti-TREM2 antibody |
| 中文名称 | 髓系细胞触发受体2抗体 |
| 别 名 | Triggering receptor expressed on myeloid cells 2; TREM-2; TREM2a; TREM2b; TREM2c; Trggering receptor expressed on myeloid cells 2a; TREM2_HUMAN. |
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Specific References (5) | bs-2723R has been referenced in 5 publications.
[IF=7.561] Elizabeth Olmsted-Davis. et al. A Population of M2 Macrophages Associated With Bone Formation. Front Immunol. 2021; 12: 686769 FC ; Mouse.
[IF=6.986] Dai Shi. et al. Synthesis and Evaluation of 68Ga-NOTA-COG1410 Targeting to TREM2 of TAMs as a Specific PET Probe for Digestive Tumor Diagnosis. Anal Chem. 2022;94(9):3819–3830 WB,IF,IHC ; Mouse,Human.
[IF=4.39] Satoh, Jun-ichi, et al. "A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues." Alzheimer's research & therapy 5.4 (2013): 30. WB ; Human.
[IF=4.12] Rai et al. Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine. (2016) Arthritis.Res.The. 18:203 IF ; Pig.
[IF=1.936] Li et al. TREM2 inhibits inflammatory responses in mouse microglia by suppressing the PI3K/NF-κB signaling. (2018) Cell.Biol.Int. Apr 16. IF&WB ;
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| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25kDa |
| 检测分子量 | 35-40 |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TREM2 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] Function: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Subunit: Interacts with TYROBP/DAP12. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). Isoform 3: Secreted (Potential). Tissue Specificity: Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. DISEASE: Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q9NZC2 Gene ID: 54209 Database links: Entrez Gene: 54209 Human Entrez Gene: 83433 Mouse Omim: 605086 Human SwissProt: Q9NZC2 Human SwissProt: Q99NH8 Mouse Unigene: 435295 Human Unigene: 261623 Mouse TREM-2是近年来发现应用于诊断炎症性疾病的重要标记物,也是感染性疾病的预警指标,与细菌感染的严重程度相关。 TREM2属于免疫球蛋白超家族TREM,表达于白细胞的细胞膜表面受体,TREM-选择性地表达于中性粒细胞和部分单核细胞,它介导的信号传导通路在炎症的发生和级联放大中起重要作用。 |
| 产品图片 |
Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Mouse BV2 cell lysates
Lane 5: Mouse Lung tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Rat Cerebrum tissue lysates
Primary: Anti-TREM2 (bs-2723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
