产品中心-北京博奥森生物技术有限公司

产品编号	bs-2013R
英文名称	phospho-Nrf2 (Ser40)
中文名称	磷酸化核因子2相关因子2(Ser40)抗体
别名	Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear factor-E2 related factor2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.
	Specific References (48) \| bs-2013R has been referenced in 48 publications. [IF=9.274] Wiebke Sachs. et al. Distinct Modes of Balancing Glomerular Cell Proteostasis in Mucolipidosis Type II and III Prevent Proteinuria. J Am Soc Nephrol. 2020 Aug;31(8):1796-1814 WB,IF ; Mouse. PubMed:32641396 [IF=8.071] Ming Qi. et al. Ellagic acid ameliorates paraquat-induced liver injury associated with improved gut microbial profile. Environ Pollut. 2022 Jan;293:118572 WB ; Pig. PubMed:34838710 [IF=7.446] Jianbing Wu. et al. Design, Synthesis, and Biological Evaluation of Organic Nitrite (NO2–) Donors as Potential Anticerebral Ischemia Agents. J Med Chem. 2021;XXXX(XXX):XXX-XXX IHC ; Rat. PubMed:34292749 [IF=7.376] Ziling Wang. et al. Ginsenoside Rg1 prevents bone marrow mesenchymal stem cell senescence via NRF2 and PI3K/Akt signaling. Free Radical Bio Med. 2021 Aug;: WB ; Mouse. PubMed:34364981 [IF=7.376] Ziling Wang. et al. Ginsenoside Rg1 prevents bone marrow mesenchymal stem cell senescence via NRF2 and PI3K/Akt signaling. Free Radical Bio Med. 2021 Aug;: WB ; Mouse. PubMed:34364981 [IF=7.19] Mo, Chunfen, et al. "The Crosstalk between Nrf2 and AMPK Pathways Is Important for the Anti-inflammatory Effect of Berberine in LPS-Stimulated Macrophages and Endotoxin-Shocked Mice."?Antioxidants & redox signaling?ja (2013). PubMed:23875776 [IF=7.129] Jie Luo. et al. Copper nanoparticles lead to reproductive dysfunction by affecting key enzymes of ovarian hormone synthesis and metabolism in female rats. ECOTOX ENVIRON SAFE. 2023 Apr;254:114704 WB ; Rat. PubMed:36898311 [IF=6.922] Yunxiao Zhong. et al. Nrf2 Inhibits the Progression of Parkinson’s Disease by Upregulating AABR07032261.5 to Repress Pyroptosis. J Inflamm Res. 2022 Feb;15:669-685 WB ; Rat. PubMed:35140498 [IF=6.819] Kim, Da-Eun. et al. Plk2-mediated phosphorylation and translocalization of Nrf2 activates anti-inflammation through p53/Plk2/p21cip1 signaling in acute kidney injury. CELL BIOL TOXICOL. 2022 Jul;:1-21 WB ; Rat. PubMed:35842499 [IF=6.656] Ye Yang. et al. Neocryptotanshinone protects against myocardial ischemia-reperfusion injury by promoting autolysosome degradation of protein aggregates via the ERK1/2-Nrf2-LAMP2 pathway. PHYTOMEDICINE. 2023 Feb;110:154625 WB ; Rat. PubMed:36586206 [IF=6.388] Rui Ji. et al. Carnosol inhibits KGN cells oxidative stress and apoptosis and attenuates polycystic ovary syndrome phenotypes in mice through Keap1-mediated Nrf2/HO-1 activation. PHYTOTHER RES. 2023 Feb;: WB ; Mouse,Human. PubMed:36786429 [IF=6.205] Michelle L. Kerns. et al. Pathogenic and therapeutic role for NRF2 signaling in ultraviolet light–induced skin pigmentation. Jci Insight. 2020 Oct 15; 5(20): e139342 IF,IHC ; Human, Mouse. PubMed:33001866 [IF=5.74] Santofimia-Castaño, Patricia, et al. "Melatonin induces the expression of Nrf2-regulated antioxidant enzymes via PKC and Ca 2+ influx activation in mouse pancreatic acinar cells." Free Radical Biology and Medicine (2015). Rat. PubMed:26163001 [IF=5.27] Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and Medicine WB ; Human, Mouse. PubMed:23434766 [IF=5.27] Lee, Bao-Hong, et al. "Ankaflavin: A natural novel PPAR?? agonist up-regulated Nrf2 to attenuate methylglyoxal (MG)-induced diabetes< i> in vivo."Free Radical Biology and Medicine (2012).y WB ; Rat. PubMed:23022408 [IF=5.201] Marina K et al. Mesenchymal Stem Cells Early Response to Low-Dose Ionizing RadiationFront Cell Dev Biol.2020 Dec 14;8:584497. FCM ; Human. PubMed:33381502 [IF=5.187] Ana L. Vivinetto. et al. Myelin-associated glycoprotein activation triggers glutamate uptake by oligodendrocytes in vitro and contributes to ameliorate glutamate-mediated toxicity in vivo. Bba-Mol Basis Dis. 2022 Apr;1868:166324 IF ; Rat. PubMed:34954343 [IF=5.03] Yen, Ting-Lin, et al. "Andrographolide stimulates p38 MAPK-Nrf2-HO-1 signaling in primary cerebral endothelial cells for definite protection against ischemic stroke in rats." Translational Research (2016). WB ; Mouse. PubMed:26746802 [IF=4.925] Xueling Zhang. et al. Type 2 Diabetes Mellitus Makes Corneal Endothelial Cells Vulnerable to Ultraviolet A-Induced Oxidative Damage Via Decreased DJ-1/Nrf2/NQO1 Pathway. INVEST OPHTH VIS SCI. 2022 Nov;63(12):25-25 WB ; Human. PubMed:36383351 [IF=4.716] Gong H et al. Nrf2-SHP Cascade-Mediated STAT3 Inactivation Contributes to AMPK-Driven Protection Against Endotoxic Inflammation. Front Immunol. 2020 Mar 10;11:414. WB ; Mouse. PubMed:32210977 [IF=4.556] Jong Ik Jeonet al. Bacteroides fragilis Enterotoxin Induces Sulfiredoxin-1 Expression in Intestinal Epithelial Cell Lines Through a Mitogen-Activated Protein Kinases- and Nrf2-Dependent Pathway, Leading to the Suppression of Apoptosis. Int J Mol Sci . 2020 Jul 29;21(15):5383. WB ; Human. PubMed:32751114 [IF=4.48] Cárdeno, Ana, et al. "Squalene targets pro-and anti-inflammatory mediators and pathways to modulate over-activation of neutrophils, monocytes and macrophages." Journal of Functional Foods 14 (2015): 779-790. WB ; Human. PubMed:doi:10.1016/j.jff.2015.03.009 [IF=4.412] Ning Zhou. et al. Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2. MOLECULES. 2022 Jan;27(11):3542 WB ; Duck. PubMed:35684483 [IF=4.315] Hao Huang. et al. Maintaining blood retinal barrier homeostasis to attenuate retinal ischemia-reperfusion injury by targeting the KEAP1/NRF2/ARE pathway with lycopene. Cell Signal. 2021 Dec;88:110153 WB,IF ; Human. PubMed:10.1016/j.cellsig.2021.110153 [IF=4.29] Ghosh, Anindya, et al. "Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice." Endocrinology 158.4 (2017): 903-919. WB ; Mouse. PubMed:28324005 [IF=4.119] Cheng-You Lu. et al. Calycosin alleviates H2O2-induced astrocyte injury by restricting oxidative stress through the Akt/Nrf2/HO-1 signaling pathway. 2022 Jan 06 WB ; Rat. PubMed:34990515 [IF=4.109] Jhong-Kuei Chen. et al. Artemisia argyi extract ameliorates IL-17A-induced inflammatory response by regulation of NF-κB and Nrf2 expression in HIG-82 synoviocytes. ENVIRON TOXICOL. 2022 Aug;: WB ; Rabbit. PubMed:35959841 [IF=3.961] Ghosh et al. Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice. (2017) Endocrinology. 158:903-919 WB ; Mouse. PubMed:28324005 [IF=3.85] Kim, Mi-Hwi, et al. "EX4 stabilizes and activates Nrf2 via PKCδ, contributing to the prevention of oxidative stress-induced pancreatic beta cell damage." Toxicology and Applied Pharmacology (2016). WB ; Rat. PubMed:27939242 [IF=3.73] Ko, Su Hyuk, et al. "Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinases-and NF-κB-Dependent Pathway, Leading to Modulation of Apoptosis." Infection and Immunity (2016): IAI-00191. IF(ICC) ; Mouse. PubMed:27324483
产品类型	磷酸化抗体
研究领域	免疫学染色质和核信号细胞凋亡转录调节因子激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat,Pig (predicted: Chicken,Dog,Cow)
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg /test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	68kDa
细胞定位	细胞核细胞浆
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40: DF(p-S)QR
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors. Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit: Heterodimer. Forms a ternary complex with PGAM5 and KEAP1. May bind DNA with an unknown protein. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Post-translational modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. SWISS: Q16236 Gene ID: 4780 Database links: Entrez Gene: 4780 Human Omim: 600492 Human SwissProt: Q16236 Human
产品图片	Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: MCF-7 (Human) Cell Lysate at 30 ug Lane 4: Jurkat (Human) Cell Lysate at 30 ug Lane 5: B16 (Human) Cell Lysate at 30 ug Lane 6: A549 (Human) Cell Lysate at 30 ug Primary: Anti-phospho-Nrf2 (Ser40) (bs-2013R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 135 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (bs-2013R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-Nrf2 (Ser40)) Polyclonal Antibody, Unconjugated (bs-2013R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (bs-2013R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-Nrf2 (Ser40) antibody (bs-2013R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

	免疫组化常用试剂
	免疫印迹常用试剂
	各种标记二抗

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序

	Alexa Fluor 350 标记
	Alexa Fluor 488 标记
	Alexa Fluor 555 标记
	Alexa Fluor 594 标记
	Alexa Fluor 647 标记
	AP 标记
	APC 标记
	Biotin 标记
	Cy3 标记
	Cy5 标记
	Cy5.5 标记
	Cy7 标记
	FITC 标记
	Gold 标记
	HRP 标记
	PE 标记
	PE-Cy3 标记
	PE-CY5 标记
	PE-CY5.5 标记
	PE-CY7 标记
	RBITC 标记