| 产品编号 | bs-2231R |
| 英文名称 | Rabbit Anti-LYRIC antibody |
| 中文名称 | 星形胶质细胞升高基因1抗体 |
| 别 名 | Astrocyte elevated gene 1; AEG 1; AEG1; MTDH; AEG1; AEG-1; LYRIC; Protein LYRIC; Lysine-rich CEACAM1 co-isolated protein; 3D3/LYRIC; Metastasis adhesion protein; Metadherin; Astrocyte elevated gene-1 protein; 3D3; Lysine rich CEACAM1 associated protein |
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Specific References (4) | bs-2231R has been referenced in 4 publications.
[IF=2.52] Zheng, Jiasheng, et al. ʺAstrocyte elevated gene-1 (AEG-1) shRNA sensitizes Huaier polysaccharide (HP)-induced anti-metastatic potency via inactivating downstream P13K/Akt pathway as well as augmenting cell-mediated immune response.ʺ Tumor Biology (2013): 1-6. WB ; Human.
[IF=2.11] Zhang, Chun, et al. "MTDH/AEG-1-based DNA vaccine suppresses metastasis and enhances chemosensitivity to paclitaxel in pelvic lymph node metastasis."Biomedicine & Pharmacotherapy (2015). IHC-P ; Mouse.
[IF=1.37] Guo, Feng, et al. "Astrocyte elevated gene-1 overexpression in histologically favorable Wilms tumor is related to poor prognosis." Journal of Pediatric Urology (2013). Human.
[IF=0] Guo, Jia, et al. "AEG-1 expression correlates with CD133 and PPP6c levels in human glioma tissues." The Journal of Biomedical Research (2014). IHC-P ; Human.
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| 研究领域 | 肿瘤 信号转导 上皮细胞 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 64kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AEG-1: 131-230/582 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumour tissues. Function: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. Subunit: Interacts with BCIPP, CREBBP/CBP and RELA/p65. Subcellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction. Nucleus, nucleolus . Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain. TNF-alpha causes translocation from the cytoplasm to the nucleus. Tissue Specificity: Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. SWISS: Q86UE4 Gene ID: 92140 Database links: Entrez Gene: 92140 Human Entrez Gene: 67154 Mouse Omim: 610323 Human SwissProt: Q86UE4 Human SwissProt: Q80WJ7 Mouse Unigene: 377155 Human Unigene: 130883 Mouse Unigene: 6864 Rat |
| 产品图片 |
Sample:
A549(Human) Lysate at 40 ug
Primary: Anti-LYRIC (bs-2231R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LYRIC) Polyclonal Antibody, Unconjugated (bs-2231R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-LYRIC antibody (bs-2231R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
