| 产品编号 | bs-2698R |
| 英文名称 | Rabbit Anti-MCT4/SLC16A3 antibody |
| 中文名称 | 单羧酸转运蛋4抗体 |
| 别 名 | Monocarboxylate transporter 4; Slc16a3; Mct3; Mct4; MCT-4; Monocarboxylate transporter 3; solute carrier family 16 member 3; SLC16A3; MOT4_HUMAN. |
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Specific References (6) | bs-2698R has been referenced in 6 publications.
[IF=6.78] Pedro Paulo Menezes Scariot. et al. Living high - training low model applied to C57BL/6J mice: Effects on physiological parameters related to aerobic fitness and acid-base balance. LIFE SCI. 2023 Mar;317:121443 WB ; Mouse.
[IF=6.78] P.P.M. Scariot. et al. Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training. LIFE SCI. 2022 Oct;307:120872 WB ; Mouse.
[IF=4.398] Qingxian Li. et al. Glucocorticoid caused lactic acid accumulation and damage in human chondrocytes via ROS-mediated inhibition of Monboxylate Transporter 4. Bone. 2021 Dec;:116299 WB,IF,IHC ; Rat.
[IF=3.905] Xiao Han. et al. ZEB1 induces ROS generation through directly promoting MCT4 transcription to facilitate breast cancer. Exp Cell Res. 2022 Mar;412:113044 IHC ; Human.
[IF=3.421] Ruofan Yao. et al. Maternal obesity-associated disruption of polarized lactate transporter MCT4 expression in human placenta. REPROD TOXICOL. 2022 Sep;112:1 WB, IF ; Human.
[IF=2.323] Carolina Manosalva. et al. D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFκB Pathways. Animals-Basel. 2020 Nov;10(11):2105 WB ; Bovine.
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| 研究领域 | 肿瘤 细胞生物 转录调节因子 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC16A3: 401-465/465 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM, Mar 2008] Function: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Highly expressed in skeletal muscle. Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. SWISS: O15427 Gene ID: 9123 Database links: Entrez Gene: 9123 Human Entrez Gene: 80879 Mouse Omim: 603877 Human SwissProt: O15427 Human SwissProt: P57787 Mouse Unigene: 500761 Human Unigene: 28632 Mouse Unigene: 10826 Rat |
| 产品图片 |
Sample: Muscle (Mouse) Lysate at 30 ug
Primary: Anti-MCT4(bs-2698R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Sample: HepG2 Cell Lysate at 30 ug
Primary: Anti-MCT4(bs-2698R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Rat Muscle tissue lysates
Primary: Anti-MCT4/SLC16A3 (bs-2698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCT4) Polyclonal Antibody, Unconjugated (bs-2698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MCT4 Polyclonal Antibody, Unconjugated(bs-2698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
