产品编号 | bs-2688R |
英文名称 | Rabbit Anti-ApoA4 antibody |
中文名称 | 载脂蛋白A4抗体 |
别 名 | Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4; APOA4; MGC142154; MGC142156; APOA4_HUMAN. |
研究领域 | 细胞生物 免疫学 细胞膜受体 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Dog,Horse,Rabbit) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 41kDa |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ApoA4: 21-120/396 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq]. Subcellular Location: Secreted. Tissue Specificity: Synthesized primarily in the intestine and secreted in plasma. Similarity: Belongs to the apolipoprotein A1/A4/E family. SWISS: P06727 Gene ID: 337 Database links: Entrez Gene: 337 Human Omim: 107690 Human SwissProt: P06727 Human Unigene: 1247 Human |
产品图片 |
Sample:
Lane 1: Kidney (Rat) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Primary: Anti-ApoA4 (bs-2688R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ApoA4) Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ApoA4) Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |