| 产品编号 | bs-2684R |
| 英文名称 | GPNMB Rabbit pAb |
| 中文名称 | GPNMB抗体 |
| 别 名 | HGFIN; NMB; PLCA3; DC-HIL; Dchil; ipd; GPNMB_HUMAN; GPNMB; Hematopoietic growth factor inducible neurokinin-1 type; GPNMB_MOUSE; Dendritic cell-associated transmembrane protein; Osteoactivin; GPNMB_RAT; |
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Specific References (9) | bs-2684R has been referenced in 9 publications.
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| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Pig,Cow,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPNMB: 81-180/572 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Could be a melanogenic enzyme. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Not restricted to the melanocytic lineage. Similarity: Belongs to the PMEL/NMB family. Contains 1 PKD domain. SWISS: Q14956 Gene ID: 10457 Database links: Entrez Gene: 10457 Human Entrez Gene: 93695 Mouse Omim: 604368 Human SwissProt: Q14956 Human SwissProt: Q99P91 Mouse Unigene: 190495 Human Unigene: 302602 Mouse Unigene: 13778 Rat |
| 产品图片 |
Sample:
Lane 1: Recombinant human GPNMB protein, C-His (HEK293)(bs-43555P)
Primary: Anti-GPNMB (bs-2684R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa
Tissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(bs-2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
