| 产品编号 | bs-2679R |
| 英文名称 | CD112 |
| 中文名称 | 细胞粘附分子CD112抗体 |
| 别 名 | Nectin-2; Nectin2; Nectin 2; CD 112; CD112 antigen; Herpes virus entry mediator B; Herpes virus entry protein B; Herpesvirus entry mediator B; Herpesvirus entry protein B; Hve B; HveB; Poliovirus receptor like 2; Poliovirus receptor related 2 (herpesvirus entry mediator B); Poliovirus receptor related 2; Poliovirus receptor related protein 2; Poliovirus receptor-related protein 2; Nectin cell adhesion molecule 2; HVEB; PRR 2; PRR2; PVRL 2; PVRL2; NECT2_HUMAN; PVRR 2; PVRR2. |
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Specific References (1) | bs-2679R has been referenced in 1 publications.
[IF=1.45] Waters, Alicia M., et al. "Effect of Repeat Dosing of Engineered Oncolytic Herpes Simplex Virus on Preclinical Models of Rhabdomyosarcoma." Translational Oncology 9.5 (2016): 419-430. WB ; Human.
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| 研究领域 | 肿瘤 免疫学 神经生物学 细胞粘附分子 细胞表面分子 内皮细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD112: 251-350/538 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Nectin 2 is a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Can form trans-heterodimers with PVRL3/nectin-3. Function: Probable cell adhesion protein. Subunit: Can form trans-heterodimers with PVRL3/nectin-3. Interacts with CD226. Binds with low affinity to TIGIT. Interacts with herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV) envelope glycoprotein D; functions as an entry receptor for these viruses. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the nectin family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q92692 Gene ID: 5819 Database links: Entrez Gene: 5819 Human Entrez Gene: 19294 Mouse Omim: 600798 Human SwissProt: Q92692 Human SwissProt: P32507 Mouse Unigene: 655455 Human Unigene: 4341 Mouse Unigene: 203297 Rat 细胞粘附分子Nectin-2（CD112）是一种同源性非钙依赖型的CAM，属免疫球蛋白超家族，由nectin-1、nectin-2、nectin-3组成，广泛存在于肿瘤、正常细胞，神经细胞、内皮细胞等，CD112也是单纯疱疹病毒(HSV-1和HSV-2)的受体，具有同型或异型细胞粘附活性.在多发性硬化中呈现高表达。 |
| 产品图片 |
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD112 antibody(bs-2679R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); econdary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
