| 产品编号 | bs-2612R |
| 英文名称 | IL17D Rabbit pAb |
| 中文名称 | 白介素-17D抗体 |
| 别 名 | IL17D_HUMAN; IL17D; IL-17D; Interleukin-27 (IL-27); |
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Specific References (2) | bs-2612R has been referenced in 2 publications.
[IF=5.714] Tianming Li. et al. IL-17D affects the chemokines and chemokine receptors of intestinal epithelial cells under hyperoxia. INT IMMUNOPHARMACOL. 2022 Dec;113:109386
[IF=3.098] Xuying Liu. et al. Involvement of nuclear factor erythroid 2‑related factor 2 in neonatal intestinal interleukin‑17D expression in hyperoxia. Int J Mol Med. 2020 Oct;46(4):1423-1432 WB,IF,IHC ; Rat.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-17D: 31-130/202 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008] Function: Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells. Subcellular Location: Secreted. Tissue Specificity: Expressed preferentially in adipose, skeletal muscle and CNS. Similarity: Belongs to the IL-17 family. SWISS: Q8TAD2 Gene ID: 53342 Database links: Entrez Gene: 53342 Human Entrez Gene: 239114 Mouse Omim: 607587 Human SwissProt: Q8TAD2 Human Unigene: 655142 Human |
| 产品图片 |
Sample:
Jurkat (Human) Cell Lysate at 40 ug
Primary: Anti-IL17D (bs-2612R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL17D) Polyclonal Antibody, Unconjugated (bs-2612R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL17D) Polyclonal Antibody, Unconjugated (bs-2612R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
