| 产品编号 | bs-2575R |
| 英文名称 | Glycophorin A Rabbit pAb |
| 中文名称 | 血型糖蛋白A（CD235a）抗体 |
| 别 名 | CD235a; GPA; GPErik; GPSAT; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2; GLPA_HUMAN; GYPA; MN sialoglycoprotein; Sialoglycoprotein alpha; GLPA_MOUSE; |
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Specific References (1) | bs-2575R has been referenced in 1 publications.
[IF=6.8] Khan, Mohammad Badruzzaman. et al. Exercise Improves Cerebral Blood Flow and Functional Outcomes in an Experimental Mouse Model of Vascular Cognitive Impairment and Dementia (VCID). TRANSL STROKE RES. 2023 Jan;:1-16 FCM ; Mouse.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse GPA: 74-150/150 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008] Function: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Subunit: Homodimer. Interacts with Streptococcus gordonii has protein. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Appears to be colocalized with SLC4A1. Similarity: Belongs to the glycophorin A family. SWISS: P14220 Gene ID: 14934 Database links: Entrez Gene: 2993 Human Entrez Gene: 14934 Mouse Omim: 111300 Human SwissProt: P02724 Human SwissProt: P14220 Mouse Unigene: 434973 Human Unigene: 13123 Mouse |
| 产品图片 |
Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Placenta (Mouse) Lysate at 40 ug
Lane 5: Spleen (Mouse) Lysate at 40 ug
Primary:
Anti-Glycophorin A (bs-2575R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: HL60 (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: Jurkat (Human) Cell Lysate at 30 ug
Lane 5: 293T (Human) Cell Lysate at 30 ug
Primary:
Anti-Glycophorin A (bs-2575R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Western blot analysis of extracts from bone using Glycophorin antibody. (Primary dilution:1:300)
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
