| 产品编号 | bs-2574R |
| 英文名称 | Rabbit Anti-GCSF Receptor antibody |
| 中文名称 | 粒细胞-巨噬细胞集落刺激因子3受体抗体 |
| 别 名 | GCSF Receptor; CD 114; CD114; CD114 antigen; Colony stimulating factor 3 receptor (granulocyte); Colony stimulating factor 3 receptor; CSF 3R; CSF3R; CSF3R_HUMAN; Csfgr; G CSF R; G-CSF receptor; G-CSF-R; G-CSFR; GCSFR; Granulocyte colony stimulating factor receptor; Granulocyte colony stimulating factor receptor precursor; Granulocyte colony-stimulating factor receptor. |
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Specific References (5) | bs-2574R has been referenced in 5 publications.
[IF=4.671] Hong L et al. Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage. J Mol Cell Biol. 2019 Nov 19. pii: mjz105. WB ; Mouse.
[IF=4] Hong L et al. Protective effects of Human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage. J Mol Cell Biol. 2020 Jun 11;12(5):372-385. WB ; Human.
[IF=3.777] Jia-hui Che. et al. Macrophage Polarization in Placenta Accreta and Macrophage-trophoblast Interactions. AM J REPROD IMMUNOL. 2022 Aug;: IHC ; Human.
[IF=1.78] Tajima, Shogo, et al. "Case Report Granulocyte colony-stimulating factor-producing undifferentiated carcinoma of the colon mimicking a pulmonary giant cell carcinoma: a case showing overexpression of CD44 along with highly proliferating." Int J Clin Exp Pathol 7.10 (2014): 7034-7041. IHC-P ; Human.
[IF=1.69] Zhang, Enji, et al. "Expression of granulocyte colony-stimulating factor 3 receptor in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain." Molecular Medicine Reports (2017). WB, IHC-P ; Rat, Human.
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| 研究领域 | 细胞生物 免疫学 发育生物学 信号转导 干细胞 生长因子和激素 细胞膜受体 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 95kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G-CSFR: 101-200/863 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by the GCSF Receptor gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, of which three are membrane-bound and the one secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.
Function: Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. Subunit: Homodimer. The dimeric receptor binds two CSF3 molecules. Subcellular Location: Isoform 2: Secreted (Probable). Cell membrane; Single-pass type I membrane protein. Tissue Specificity: One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U937 cells. Isoform GSCFR-3 is highly expressed in placenta. DISEASE: Defects in CSF3R are the cause of hereditary neutrophilia (NEUTROPHILIA) [MIM:162830]. A form of lifelong, persistent neutrophilia, a condition characterized by an increase in the number of neutrophils in the blood. Similarity: Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 5 fibronectin type-III domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. SWISS: Q99062 Gene ID: 1441 Database links: Entrez Gene: 1441 Human Entrez Gene: 12986 Mouse Omim: 138971 Human SwissProt: Q99062 Human SwissProt: P40223 Mouse Unigene: 524517 Human Unigene: 271701 Mouse |
| 产品图片 |
Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti- GCSF Receptor (bs-2574R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95kD
Observed band size: 120kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
