| 产品编号 | bs-2569R |
| 英文名称 | CD226 |
| 中文名称 | CD226抗体 |
| 别 名 | DNAX accessory molecule 1; adhesion glycoprotein; CD226 antigen; DNAM1; platelet and T cell activation antigen 1; PTA1; TLiSA1; CD226; CD226 molecule; CD226_HUMAN; DNAM 1; DNAM-1; T lineage specific activation antigen 1 antigen. |
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Specific References (1) | bs-2569R has been referenced in 1 publications.
[IF=15.304] Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635 WB, IF, FC ; Mouse.
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| 研究领域 | 免疫学 细胞粘附分子 细胞表面分子 细胞类型标志物 自然杀伤细胞 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD226: 201-300/336 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. [provided by RefSeq, Jul 2008] Subunit: Interacts with PVR and PVRL2. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by peripheral blood T-lymphocytes. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q15762 Gene ID: 10666 Database links: Entrez Gene: 10666 Human Omim: 605397 Human SwissProt: Q15762 Human Unigene: 660130 Human CD226分子是一种广泛表达于多种免疫细胞膜表面的Ⅰ型跨膜糖蛋白，CD226广泛表达于T细胞、NK细胞、NK T细胞、活化的血管内皮细胞、巨核/血小板谱系、单核细胞、胸腺细胞、某些B细胞亚群、部分造血干细胞和肥大细胞等免疫细胞表面，在免疫系统以外则分布于海马和小脑皮质的神经突触部位。CD226与其配体的相互作用参与CTL和NK细胞的活化、分化和细胞毒作用,调节CD4+ T细胞的增殖和分化，介导内皮细胞与其他细胞的黏附,促进血小板的活化与聚集，参与造血调控，介导肥大细胞的脱颗粒作用，参与NK T细胞和胸腺细胞的抗凋亡作用、树突状细胞的成熟、免疫突触和神经突触的形成，并与肿瘤、自身免疫性疾病、移植排斥及病毒感染等疾病密切相关。 |
| 产品图片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-CD226 (bs-2569R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD 
Blank control(blue): Jurkat cells(fixed with 2% paraformaldehyde (10 min) ).
Primary Antibody:Rabbit Anti-CD226 antibody(bs-2569R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
