产品编号 | bs-2591R |
英文名称 | Rabbit Anti-Fbx32 antibody |
中文名称 | 泛素蛋白连接酶抗体 |
别 名 | Fbx32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein. |
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Specific References (3) | bs-2591R has been referenced in 3 publications.
[IF=6.684] Ma X. et al. Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies.. Front Cell Dev Biol. 2022 Feb;10:791986-791986 IHC ; Human.
[IF=5.62] Lin, Xuzhu, et al. "Hindlimb Immobilization, but not Castration, Induces Reduction of Undercarboxylated Osteocalcin Associated with Muscle Atrophy in Rats." Journal of Bone and Mineral Research (2016). WB ; Rat.
[IF=3.412] Zhang Quan-Bing. et al. Effect of Electrical Stimulation on Disuse Muscular Atrophy Induced by Immobilization: Correlation with Upregulation of PERK Signal and Parkin-Mediated Mitophagy. AM J PHYS MED REHAB. 2023 Jan;:10.1097/PHM.0000000000002182 IHC ; Rabbit.
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研究领域 | 细胞生物 干细胞 泛素 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 42kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MAFbx: 31-130/355 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Function: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. Subunit: Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32. Subcellular Location: Cytoplasm. Nucleus. Note=Shuttles between Cytoplasm and the nucleus. Tissue Specificity: Specifically expressed in cardiac and skeletal muscle. Similarity: Contains 1 F-box domain. SWISS: Q969P5 Gene ID: 114907 Database links: Entrez Gene: 114907 Human Entrez Gene: 67731 Mouse Omim: 606604 Human SwissProt: Q969P5 Human SwissProt: Q9CPU7 Mouse Unigene: 403933 Human Unigene: 292042 Mouse Unigene: 72619 Rat |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAFbx/Fbx32 Polyclonal Antibody, Unconjugated(bs-2591R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Fbx32 antibody (bs-2591R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |