| 产品编号 | bs-2532R |
| 英文名称 | ALT1 Rabbit pAb |
| 中文名称 | 谷丙氨酸氨基转移酶1抗体 |
| 别 名 | AAT1; ALT; ALT1; GPT1; SGPT; 1300007J06Rik; 2310022B03Rik; Gpt-1; ALAT; ALAT1_BOVIN; GPT; Glutamate pyruvate transaminase 1 (GPT 1); Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; 2.6.1.2; ALAT1_HUMAN; ALAT1_MOUSE; ALAT1_RAT; |
| 研究领域 | 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Cow) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALT1: 301-400/496 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Alanine Transaminase [glutamic pyruvate transaminase - GPT] participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Pyridoxal phosphate is a known cofactor. Expression is induced by glucocorticoids. This enzyme is present in a high concentration in liver tissue and it's release into the blood indicates liver disease or damage. Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Subcellular Location: Cytoplasm. Tissue Specificity: Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue. Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. SWISS: P24298 Gene ID: 2875 Database links: Entrez Gene: 2875 Human Entrez Gene: 76282 Mouse Omim: 138200 Human SwissProt: P24298 Human SwissProt: Q8QZR5 Mouse Unigene: 103502 Human Unigene: 30130 Mouse Unigene: 6318 Rat |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-ALT1 (bs-2532R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
