| 产品编号 | bs-2523R |
| 英文名称 | CD99 |
| 中文名称 | CD99抗体 |
| 别 名 | T-cell surface glycoprotein E; T cell surface glycoprotein E2 precursor; 12E7; Antigen identified by monoclonal 12E7 Y homolog; Antigen identified by monoclonal antibodies 12E7 F21 and O13; CD 99; CD99 antigen; CD99 molecule; CD99X; E2 antigen; Ewing Sarcoma; MIC 2; MIC 2X; MIC 2Y; MIC2; MIC2X; MIC2Y; Monoclonal antibody 12E7; MSK5X; Protein MIC2; Surface antigen MIC2. |
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Specific References (1) | bs-2523R has been referenced in 1 publications.
[IF=20.565] Aguayo-Mazzucato C et al. β Cell Aging Markers Have Heterogeneous Distribution and Are Induced by Insulin Resistance. (2017) Cell.Metab. 25:898-910.e5 IF ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 细胞表面分子 糖蛋白 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 16kDa |
| 检测分子量 | 32 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD99: 45-150/175 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Involved in T-cell adhesion processes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Subunit: Homodimer. Interacts with PILRB. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Natural killer cells. Similarity: Belongs to the CD99 family. SWISS: Q8VCN6 Gene ID: 673094 Database links: Entrez Gene: 4267 Human Entrez Gene: 673094 Mouse Omim: 313470 Human SwissProt: P14209 Human SwissProt: Q8VCN6 Mouse Unigene: 653349 Human Unigene: 423040 Mouse CD99（P30/32MIC2）是一种跨膜蛋白，为MIC2基因产物。分布于淋巴细胞、胸腺皮质细胞、卵巢的颗粒细胞、胰岛细胞和睾丸支持细胞。 |
| 产品图片 |
Sample: Lung (Mouse) Lysate at 30 ug
Primary: Anti- CD99 (bs-2523R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 16 kD Observed band size: 16 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
