| 产品编号 | bs-2499R |
| 英文名称 | CD69 |
| 中文名称 | 活化诱导分子CD69抗体 |
| 别 名 | CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26;C-type lectin domain family 2 member C; CD69_HUMAN; |
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Specific References (3) | bs-2499R has been referenced in 3 publications.
[IF=12.121] Rouven Hoefflin. et al. HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice. Nat Commun. 2020 Aug;11(1):1-21 IHC ; Mouse.
[IF=10.711] Zihuan Luo. et al. Ex vivo anchored PD-L1 functionally prevent in vivo renal allograft rejection. BIOENG TRANSL MED. 2022 Apr 06 FC ; Rat.
[IF=4.12] Mokuda et al. CD1a+ survivin+ dendritic cell infiltration in dermal lesions of systemic sclerosis. (2015) Arthritis.Res.Ther. 17:275 IHC ; Human.
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| 研究领域 | 细胞生物 免疫学 干细胞 糖尿病 细胞表面分子 自然杀伤细胞 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog) |
| 产品应用 | WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 检测分子量 | 27-33 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD69: 101-199/199 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011] Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. Subunit: Homodimer; disulfide-linked. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. Post-translational modifications: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. Similarity: Contains 1 C-type lectin domain. SWISS: Q07108 Gene ID: 969 Database links: Entrez Gene: 969 Human Entrez Gene: 12515 Mouse Omim: 107273 Human SwissProt: Q07108 Human SwissProt: P37217 Mouse Unigene: 208854 Human Unigene: 74745 Mouse Unigene: 23779 Rat CD69属于C-型凝集素受体家族,也是NK细胞信号传导基因复合体家族的一员,是一种Ⅱ型跨膜糖蛋白. |
| 产品图片 |
Sample: U937 Cell Lysate at 30 ug
Primary: Anti- CD69 (bs-2499R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 34 kD 
Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug Lane 2: Spleen (Mouse) Lysate at 40 ug Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33/27 kD Observed band size: 30 kD 
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) .
Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
