| 产品编号 | bs-2486R |
| 英文名称 | Rabbit Anti-SLAMF9 antibody |
| 中文名称 | CD2F-10抗体 |
| 别 名 | SLAM family member 9; CD2 family member 10; CD2F-10; CD84 homolog 1; CD84-H1; 2310026I04Rik; AI462096; CD84-H1; MGC88194; SF2001; slamf9; SLAMF9; SLAF9_HUMAN. |
| 研究领域 | 细胞生物 免疫学 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLAMF9: 51-150/289 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the signaling lymphocytic activation molecule family. May play a role in the immune response. The encoded protein is a cell surface molecule that consists of two extracellular immunoglobulin domains, a transmembrane domain and a short cytoplasmic tail that lacks the signal transduction motifs found in other family members. Alternative splicing results in multiple transcript variants. Expression is predominantly restricted in hematopoietic tissues. Expressed in heart, spleen, liver, intestine, muscle and testis. Expressed in immune cells, including monocytes, dendritic, B- and T-cells. No expression was seen in peripheral blood leukocytes. Expressed in the leukocyte cell line THP1-macrophage. Function: May play a role in the immune response. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expression is predominantly restricted in hematopoietic tissues. Expressed in heart, spleen, liver, intestine, muscle and testis. Expressed in immune cells, including monocytes, dendritic, B- and T-cells. No expression was seen in peripheral blood leukocytes. Expressed in the leukocyte cell line THP1-macrophage. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. SWISS: Q96A28 Gene ID: 89886 Database links: Entrez Gene: 89886 Human Entrez Gene: 98365 Mouse Omim: 608589 Human SwissProt: Q96A28 Human SwissProt: Q9D780 Mouse Unigene: 661712 Human Unigene: 150050 Mouse |
| 产品图片 |
Sample:
A549 (human)cell Lysate at 40 ug
BV2 (mouse)cell Lysate at 40 ug
Primary: Anti- SLAMF9 (bs-2486R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30kD
Observed band size: 35 kD
Blank control: (mo)splenocytes(blue)
Isotype Control Antibody: Rabbit IgG-FITC(orange) ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (rad line): Rabbit Anti-SLAMF9 antibody (bs-2486R) ;
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (grenn line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
