| 产品编号 | bs-2474R |
| 英文名称 | Rabbit Anti-CD93 antibody |
| 中文名称 | CD93抗体 |
| 别 名 | C1qrp; Complement component C1q receptor; C1q/MBL/SPA receptor; C1qR(p); C1qr1; C1qRp; CD93 antigen; CD93 molecule; CDw93; Complement component 1 q subcomponent receptor 1; Complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix remodeling associated protein 4; MXRA4. |
| 研究领域 | 免疫学 细胞粘附分子 细胞表面分子 糖蛋白 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD93: 601-652/652 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. [provided by RefSeq, Jul 2008]. Function: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. Subunit: Interacts with HCV core protein. Interacts with C1QBP; the association may represent a cell surface C1q receptor. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin. Post-translational modifications: N- and O-glycosylated. Similarity: Contains 1 C-type lectin domain. Contains 5 EGF-like domains. SWISS: Q9NPY3 Gene ID: 22918 Database links: Entrez Gene: 22918 Human Entrez Gene: 17064 Mouse Omim: 120577 Human SwissProt: Q9NPY3 Human SwissProt: O89103 Mouse Unigene: 97199 Human Unigene: 681 Mouse Unigene: 162695 Rat |
| 产品图片 |
Blank control:HUVEC.
Primary Antibody (green line): Rabbit Anti-CD93 antibody (bs-2474R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD93 antibody(bs-2474R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
