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Rabbit Anti-CLEC7A  antibody (bs-2455R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-2455R
英文名称 CLEC7A
中文名称 β葡聚糖受体抗体
别    名 CLC7A_HUMAN; C-type lectin domain family 7 member A; BGR; Beta GR; CLECSF12; DECTIN1; Beta-glucan receptor; beta Glucan Receptor; C-type lectin superfamily member 12; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12; Dendritic cell-associated C-type lectin 1; DC-associated C-type lectin 1; Dectin-1; Dectin 1; Dectin1; CD369; UNQ539/PRO1082;   
Specific References  (5)     |     bs-2455R has been referenced in 5 publications.
[IF=19.227] Iván Ballasch. et al. Ikzf1 as a novel regulator of microglial homeostasis in inflammation and neurodegeneration. BRAIN BEHAV IMMUN. 2023 Mar;109:144  WB ;  Mouse.  
[IF=4.784] Cui H et al. A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression. Int J Biol Macromol. 2020 Jan 23;149:801-808.  WB ;  Mouse.  
[IF=4.784] Zhan Q et al. Structural Characterization and Immunomodulatory Activity of a Novel Acid Polysaccharide Isolated from the Pulp of Rosa laevigata Michx Fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.  Other ;  Mouse.  
[IF=4.012] Wu J et al. Paeonol ameliorates murine alcohol liver disease via mycobiota‐mediated Dectin‐1/IL‐1β signaling pathway. J Leukoc Biol. 2020 Mar 4.  WB ;  mouse.  
[IF=1.16] Li et al. Expression of dectin-1 during fungus infection in human corneal epithelial cells. (2014) Int.J.Ophthalmol. 7:34-7  IHC ;  Human.  
研究领域 细胞生物  免疫学  淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-2000, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 28kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CLEC7A: 41-140/247 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq].

Function:
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity).

Subunit:
Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.

Tissue Specificity:
Detected in dendritic cells, in paracortical and medullary regions of lymph nodes, and in spleen red pulp and white pulp.

Post-translational modifications:
on tyrosine residues in response to glucan binding.

Similarity:
Contains 1 C-type lectin domain.

SWISS:
Q9BXN2

Gene ID:
64581

Database links:

Entrez Gene: 64581 Human

Entrez Gene: 56644 Mouse

Entrez Gene: 502902 Rat

Omim: 606264 Human

SwissProt: Q9BXN2 Human

SwissProt: Q6QLQ4 Mouse

Unigene: 143929 Human

Unigene: 239516 Mouse



β-葡聚糖受体是一种非调理素依赖性受体,可在免疫反应早期,在清除衰老细胞和防御感染方面发挥重要作用,进而实现其抗肿瘤、抗感染等生物学效应。
产品图片
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-beta Glucan Receptor (bs-2455R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28,47 kDa
Sample:
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- beta Glucan Receptor (bs-2455R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample: Lung(Mouse) lysate at 30ug;
Primary: Anti-beta Glucan Receptor (bs-2455R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution;
Predicted band size: 28 kD
Observed band size: 28 kD
Blank control:RAW264.7.
Primary Antibody (green line): Rabbit Anti-Iba1 antibody (bs-2455R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line):Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (bs-2455R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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