| 产品编号 | bs-2159R |
| 英文名称 | Rabbit Anti-Glypican 4 antibody |
| 中文名称 | 磷脂酰基醇蛋白聚糖-4抗体 |
| 别 名 | dJ900E8.1 glypican 4; Glypican proteoglycan 4; Gpc4; K Glypican; Kglypican; OTTHUMP00000024052; Secreted glypican 4; GPC4_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 干细胞 细胞凋亡 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57/61kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glypican 4: 351-450/556 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008] Function: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity). Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side (By similarity). Secreted glypican-4: Secreted, extracellular space (By similarity). Similarity: Belongs to the glypican family. SWISS: O75487 Gene ID: 2239 Database links: Entrez Gene: 2239 Human Entrez Gene: 14735 Mouse Omim: 300168 Human SwissProt: O75487 Human SwissProt: P51655 Mouse Unigene: 58367 Human Unigene: 1528 Mouse Unigene: 478164 Mouse |
| 产品图片 |
Blank control(blue): U-87MG (fixed with 2% paraformaldehyde (10 min). Primary Antibody:Rabbit Anti-Glypican 4 antibody(bs-2159R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
