| 产品编号 | bs-2431R |
| 英文名称 | Rabbit Anti-TNFSF13B antibody |
| 中文名称 | 肿瘤坏死因子配体超家族成员13B抗体 |
| 别 名 | Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; B cell Activating Factor; B lymphocyte stimulator; ApoL related ligand TALL 1; BAFF; Delta BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related ; eukocyte expressed ligand 1; TALL-1; CD257; CD 257; CD257 antigen; Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form; ZTNF4; TN13B_MOUSE; TN13B_HUMAN. |
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Specific References (1) | bs-2431R has been referenced in 1 publications.
[IF=9.423] Zewen Kelvin Tuong. et al. Resolving the immune landscape of human prostate at a single-cell level in health and cancer. Cell Rep. 2021 Dec;37:110132 IF ; Human,Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 生长因子和激素 激酶和磷酸酶 细胞分化 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=3μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse TNFSF13B: 221-309/309 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. [provided by RefSeq]. Function: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Subunit: Belongs to the tumor necrosis factor family. Subcellular Location: Secreted and Cell membrane. Tissue Specificity: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Post-translational modifications: The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. Similarity: Belongs to the tumor necrosis factor family. SWISS: Q9WU72 Gene ID: 24099 Database links: Entrez Gene: 10673 Human Entrez Gene: 24099 Mouse Omim: 603969 Human SwissProt: Q9Y275 Human SwissProt: Q9WU72 Mouse Unigene: 525157 Human Unigene: 596110 Human Unigene: 28835 Mouse |
| 产品图片 |
Sample:
Lane 1: Recombinant human TNFSF13B protein, N-His(bs-43649P)
Primary: Anti-TNFSF13B (bs-2431R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 52 kDa
Blank control(blue). : Jurkat cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-BAFF antibody(bs-2431R), Dilution: 3μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Measured by its binding ability in a indirect ELISA. Immobilized TNFSF13B Peptides(Cat. bs-2431P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-TNFSF13B Antibody, the EC50 is 797.5 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
