| 产品编号 | bs-2425R |
| 英文名称 | Apelin Rabbit pAb |
| 中文名称 | 脂肪炎症因子Apelin抗体 |
| 别 名 | AGTRL1 ligand; APEL; APEL_HUMAN; Apelin-13; Apelin13; Apelin 13; APJ endogenous ligand; Apln; XNPEP2. |
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Specific References (4) | bs-2425R has been referenced in 4 publications.
[IF=4.414] Chen, Bin. et al. Apelin-13 Improves Cognitive Impairment and Repairs Hippocampal Neuronal Damage by Activating PGC-1α/PPARγ Signaling. NEUROCHEM RES. 2022 Dec;:1-12 IHC ; Mouse.
[IF=2.976] Cemre Nur Balci. et al. The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period. ANN ANAT. 2023 Feb;246:152027 WB, IHC ; Mouse.
[IF=2.915] Bülbül M et al. Acute restraint stress induces cholecystokinin release via enteric apelin.(2018) Neuropeptides. IHF ; Rat.
[IF=1.812] Kasacka et al. Comparative evaluation of cannabinoid receptors, apelin and S100A6 protein in the heart of women of different age groups. (2018) BMC.Cardiovasc.Disord. 18:190 IHC ; Human.
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| 研究领域 | 心血管 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 10 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Apelin: 65-77/77 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism. Function: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Subcellular Location: Secreted. Tissue Specificity: Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk. Post-translational modifications: Several active peptides may be produced by proteolytic processing of the peptide precursor. Similarity: Belongs to the apelin family. SWISS: Q9ULZ1 Gene ID: 8862 Database links: Entrez Gene: 8862 Human Entrez Gene: 30878 Mouse Omim: 300297 Human SwissProt: Q9ULZ1 Human SwissProt: Q9R0R4 Mouse Unigene: 303084 Human Unigene: 29262 Mouse Unigene: 22847 Rat 脂肪炎症因子apelin是血管紧张素Ⅱ-1型受体相关蛋白的天然内源性配体，是新发现的小分子活性多肽，为孤儿G蛋白偶联受体一血管紧张素受体AT-1相关蛋白(APJ)的天然配体具有调节心血管系统稳态、扩张血管、降低血压、调节免疫等多种生物学作用。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
