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Rabbit Anti-Ki-67 antibody (bs-2130R)
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说明书: 50ul  100ul  200ul
20ul/380.00元
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-2130R
英文名称 Ki-67
中文名称 Ki67蛋白抗体
别    名 Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki-67; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.  
Specific References  (20)     |     bs-2130R has been referenced in 20 publications.
[IF=3.54] Thompson, Christopher, et al. "Loss of caveolin-1 alters extracellular matrix protein expression and ductal architecture in murine mammary glands." PLOS ONE 12.2 (2017): e0172067.  IHC-P ;  Mouse.  
[IF=8.31] Li, Ning, et al. "Amphiphilic peptide dendritic copolymer-doxorubicin nanoscale conjugate self-assembled to enzyme-responsive anti-cancer agent." Biomaterials (2014).  IHC-P ;  Mouse.  
[IF=3.79] Luo, Xiaoming, et al. "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." International Journal of Pharmaceutics (2014).  IHC-P ;  Mouse.  
[IF=8.31] Pan, Dayi, et al. "PEGylated dendritic diaminocyclohexyl-platinum (II) conjugates as pH-responsive drug delivery vehicles with enhanced tumor accumulation and antitumor efficacy." Biomaterials (2014).  IHC-P ;  Mouse.  
[IF=4.75] Zhao, Yuechao, et al. "Estrogen-Induced CCN1 is Critical for Establishment of Endometriosis-like Lesions in Mice." Molecular Endocrinology (2014).  IHC-P ;  Mouse.  
[IF=4.20] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.  WB ;  Mouse.  
[IF=2.68] Yang, Fangfang, et al. "Hydroxysafflor yellow A inhibits angiogenesis of hepatocellular carcinoma via blocking ERK/MAPK and NF-κB signaling pathway in H22 tumor-bearing mice." European Journal of Pharmacology(2015).  IHC-P ;  Mouse.  
[IF=2.43] Zhang, Jing-Yao, et al. "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." World Journal of Gastroenterology 21.14 (2015): 4195-4209.  IHC-P ;  Mouse.  
[IF=4.65] Olianas, Maria C., Simona Dedoni, and Pierluigi Onali. "Antidepressants activate the lysophosphatidic acid receptor LPA 1 to induce insulin-like growth factor-I receptor transactivation, stimulation of ERK1/2 signaling and cell proliferation in CHO-K1 fibroblasts." Biochemical Pharmacology (2015).  
[IF=0.00] Sondag, Gregory R. OSTEOACTIVIN AND CD44: A NOVEL INTERACTION REGULATING BONE CELL DIFFERENTIATION AND FUNCTION. Diss. Kent State University, 2015.  IF(ICC) ;  Mouse.  
[IF=0.66] Aslaner, Arif, et al. "Medical ozone treatment ameliorates the acute distal colitis in rat." Acta Cirurgica Brasileira 31.4 (2016): 256-263.  IHC-P ;  Rat.  
[IF=5.04] Drel, Viktor R., et al. "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." The FASEB Journal(2016): fj-201500188R.  IHC-P ;  Rat.  
[IF=1.11] Kim, Ki Hyun, et al. "The oncoprotein, gankyrin, is up-regulated in middle ear cholesteatoma." Acta Oto-Laryngologica (2014): 1-6.  IHC-P ;  Human.  
[IF=2.07] Ma, Ying-Yu, et al. "Involvement of c-KIT mutation in the development of gastrointestinal stromal tumors through proliferation promotion and apoptosis inhibition." OncoTargets and Therapy 7 (2014): 637-643.  IHC-P ;  Human.  
[IF=3.73] Ye, Qiurong, et al. "Lactoferrin Deficiency Promotes Colitis-Associated Colorectal Dysplasia in Mice." PloS one 9.7 (2014): e103298.  IHC-P ;  Mouse.  
[IF=3.85] Luo, Xiaoming, et al. "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." European Journal of Pharmaceutics and Biopharmaceutics (2015).  IHC-P ;  Mouse.  
[IF=4.46] Zhao, Yuechao, et al. "Multiple Beneficial Roles of Repressor of Estrogen Receptor Activity (REA) in Suppressing the Progression of Endometriosis."Endocrinology (2016): en-2015.  IHC-P ;  Human.  
[IF=1.27] Ma, Wei‑Yuan, Kun Jia, and Yan Zhang. "IL-17 promotes keratinocyte proliferation via the downregulation of C/EBPα." Experimental and Therapeutic Medicine.  IHC-P ;  Human.  
[IF=5.74] Antognelli, Cinzia, et al. "Glyoxalase I drives epithelial-to-mesenchymal transition via argpyrimidine-modified Hsp70, miR-21 and SMAD signalling in human bronchial cells BEAS-2B chronically exposed to crystalline silica Min-U-Sil 5: transformation into a neoplastic-like phenotype." Free Radical Biology and Medicine (2016).  Human.  
[IF=6.03] Li, Xin, Xiumei Zhu, and Liyan Qiu. "Constructing aptamer anchored nanovesicles for enhanced tumor penetration and cellular uptake of water soluble chemotherapeutics." Acta Biomaterialia (2016).  IHC-P ;  Mouse.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  细胞生物  信号转导  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 358kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Ki67:1201-1300 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Proliferation Marker

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Function:
Thought to be required for maintaining cell proliferation.

Subcellular Location:
Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Similarity:
Contains 1 FHA domain.

SWISS:
P46013

Gene ID:
4288

Database links:

Entrez Gene: 4288 Human

Entrez Gene: 17345 Mouse

Entrez Gene: 246042 Rat

Omim: 176741 Human

SwissProt: P46013 Human

SwissProt: Q91VE6 Mouse

SwissProt: Q5RJM0 Rat

Unigene: 689823 Human

Unigene: 80976 Human

Unigene: 4078 Mouse

Unigene: 233802 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞增殖标志物(Proliferation Marker)
Ki67与PCNA一样,为细胞增殖的一种标记,在细胞凋亡中S、G2 、M期均有表达,G0期缺如。
Ki-67增殖指数高低与许多肿瘤的分化程度、浸润、转移以及预后密切相关,因此被广泛作为各种恶性肿瘤的必检项目之一。
产品图片
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA embryonic mouse bone labeled with Anti-Ki-67 (Proliferation Marker) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): Mouse brain (fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (bs-2130R),Dilution: 1μg /10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test.
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