| 产品编号 | bs-2412R |
| 英文名称 | APNG/MPG Rabbit pAb |
| 中文名称 | N-甲基嘌呤DNA糖基化酶 |
| 别 名 | MPG; N-methylpurine-DNA glycosylase isoform a; DNA 3 methyladenine glycosylase; 3 alkyladenine DNA glycosylase; AAG; ADPG; Alkyladenine DNA glycosylase; anpg; APNG; MDG; N methylpurine DNA glycosirase; CRA36.1; MDG; Mid1; PIG11; PIG16; 3MG_HUMAN. |
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Specific References (1) | bs-2412R has been referenced in 1 publications.
[IF=12.416] Zhou, Zhen. et al. Metabolism regulator adiponectin prevents cardiac remodeling and ventricular arrhythmias via sympathetic modulation in a myocardial infarction model. BASIC RES CARDIOL. 2022 Dec;117(1):1-17 WB ; Dog.
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| 研究领域 | 细胞生物 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APNG/MPG: 101-200/298 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. Function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Subunit: Belongs to the DNA glycosylase MPG family. Subcellular Location: Nucleus. Similarity: Belongs to the DNA glycosylase MPG family. SWISS: P29372 Gene ID: 4350 Database links: Entrez Gene: 4350 Human Entrez Gene: 268395 Mouse Omim: 156565 Human SwissProt: P29372 Human SwissProt: Q04841 Mouse Unigene: 459596 Human Unigene: 263161 Mouse Unigene: 482070 Mouse Unigene: 11241 Rat |
| 产品图片 |
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-MPG (bs-2412R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
