| 产品编号 | bs-1958R |
| 英文名称 | SLC10A1 |
| 中文名称 | 钠离子/牛磺胆酸共转运蛋白抗体 |
| 别 名 | Na+/taurocholate Cotransporting Polypeptide; Na(+)/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na / bile acid cotransporter; Na / taurocholate transport protein; Na(+)/taurocholate transport protein; NTCP; NTCP1; Sodium/taurocholate cotransporting polypeptide; Solute carrier family 10 member 1; Slc10a1 protein; Slc10a1; SBACT; SLC10A1; NTCP_HUMAN. |
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Specific References (4) | bs-1958R has been referenced in 4 publications.
[IF=6.876] Song Guochao. et al. Potential therapeutic action of tauroursodeoxycholic acid against cholestatic liver injury via hepatic Fxr/Nrf2 and CHOP-DR5-caspase-8 pathway. CLIN SCI. 2023 Apr;137(7):561-577 WB ; Mouse.
[IF=4.117] Tan Qin. et al. Oligomerization of the HBV/HDV functional receptor NTCP expressed in Sf9 insect cell. BBA-GEN SUBJECTS. 2022 Aug;:130224 IF ; Insect.
[IF=3.628] Jing Zhao. et al. The choleretic role of tauroursodeoxycholic acid exacerbates alpha-naphthylisothiocyanate induced cholestatic liver injury through the FXR/BSEP pathway. J APPL TOXICOL. 2023 Feb;: WB ; Mouse.
[IF=2.65] Shu-Yan Gao. et al. iTRAQ Quantitative Proteomic Analysis of Different Expressed Proteins and Signal Pathways in Bakuchiol-Induced Hepatotoxicity. EVID-BASED COMPL ALT. 2022 Sep 19;2022:2928240 WB ; Human.
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| 研究领域 | 免疫学 信号转导 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Rabbit,Horse,Dog,Rat,Mouse) |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg /test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NTCP: 21-120/349 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).[supplied by OMIM] Function: The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Liver and kidney Similarity: Belongs to the sodium:bile acid symporter family. SWISS: Q14973 Gene ID: 6554 Database links: Entrez Gene: 6554 Human Entrez Gene: 20493 Mouse Omim: 182396 Human SwissProt: Q14973 Human SwissProt: O08705 Mouse Unigene: 952 Human Unigene: 341781 Mouse Unigene: 9913 Rat NTCP（钠离子/牛磺胆酸共转运蛋白）(钠离子-牛磺胆酸共转运多肽)Na+/taurocholate Cotransporting Polypeptide是 肝脏的胆汁酸盐吸收主要是钠离子依赖性转运子NTCP介导的，主要吸收牛磺胆酸，是肝细胞特有的一种转运载体，分布在肝细胞基侧膜在肝小叶和肝的腺泡表达较多。 有学者认为：NTCP是乙肝病毒的细胞表面受体分子，是HBV和HDV的功能受体，这些研究将有待于科研人员的进一步证实。 |
| 产品图片 |
Sample: K562 (human)cell Lysate at 40 ug
Primary: Anti- SLC10A1 (bs-1958R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kD 
Sample:
Lane 1: Human HL60 cell lysates Lane 2: Human K562 cell lysates Lane 3: Human U-2 OS cell lysates Primary: Anti-SLC10A1 (bs-1958R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 52 kDa 
Blank control: 293T(blue).
Primary Antibody:Rabbit Anti-SLC10A1 antibody(bs-1958R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green); Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
