产品编号 | bs-2109R |
英文名称 | Rabbit Anti-V5 tag antibody |
中文名称 | V5-tag标签抗体 |
别 名 | V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag. |
产品类型 | 标签抗体 |
研究领域 | 免疫学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Recombinant protein |
产品应用 | WB=1:10000-100000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated V5 tag |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids, although it has also been used with a shorter 9 amino acid sequence. SWISS: N/A Gene ID: N/A 该抗体能特异性识别位于重组蛋白C端或N端的V5标签。 |
产品图片 |
Sample: Lane1: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-V5 tag (bs-2109R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |