| 产品编号 | bs-2122R |
| 英文名称 | GST tag |
| 中文名称 | GST标签抗体 |
| 别 名 | GST PI protein; Glutathione S Transferase; Recombinant human Glutathione S Transferase pi protein; Glutathione S-transferase class-mu 26 kDa isozyme; GST; Sj26 antigen; SjGST; GST-tag. GST PI 蛋白; |
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Specific References (2) | bs-2122R has been referenced in 2 publications.
[IF=17.521] Qing Li. et al. SAMD9 Promotes Postoperative Recurrence of Esophageal Squamous Cell Carcinoma by Stimulating MYH9-Mediated GSK3β/β-Catenin Signaling. Advanced Science. 2023 Feb;:2203573 Pull Down,WB ; Human.
[IF=0] Cao, Meng, et al. "The formation of intracellular nanoparticles correlates with cisplatin resistance." Science China Materials (2015): 1-9. WB ;
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| 产品类型 | 标签抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:20000-60000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant full length GST tag protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GST is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. SWISS: N/A Gene ID: N/A Database links:
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| 产品图片 |
Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4℃ overnight.
Sample:
Lane 1: GST-Tagged Fusion Protein Overexpression E.coli Lysate Primary: Anti-GST tag (bs-2122R-HRP) at 1/1000 dilution Predicted band size: 41 kDa Observed band size: 45 kDa 
Sample:
GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug Primary: Anti-GST Tag (bs-2122R) at 1/1000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD 
Sample:
Lane 1: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: GST-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-GST tag (bs-2122R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD 
Sample:
Recombinant GST Protein Lysate at 40 ug Primary: Anti-GST tag (bs-2122R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 27 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
