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[IF=5.469] Tidwell, Tia R.. et al. Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell lines. CANCER METAB. 2022 Dec;10(1):1-16  FC ;  Human.  

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[IF=4.35] Songsong Jiang. et al. A Comparison Study on the Therapeutic Effect of High Protein Diets Based on Pork Protein versus Soybean Protein on Obese Mice. FOODS. 2022 Jan;11(9):1227  WB ;  Mouse.  

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[IF=4.35] Chuang, Yao-Chung, et al. "Peroxisome proliferator activated receptors γ/mitochondrial uncoupling protein 2 signaling protects against seizure-induced neuronal cell death in the hippocampus following experimental status  Rat.  

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[IF=2.552]  Gaochun Zhu . et al. Mitochondrial uncoupling protein 2 is regulated through heterogeneous nuclear ribonucleoprotein K in lead exposure models. J Environ Sci Heal C. 2021;39(1):1-16  WB ;  Rat.  

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[IF=2.47] Bo, Jinshuang, et al. "Methylglyoxal Impairs Insulin Secretion of Pancreatic β-Cells through Increased Production of ROS and Mitochondrial Dysfunction Mediated by Upregulation of UCP2 and MAPKs." Journal of Diabetes Research(2015).  WB ;  Mouse.  

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Sample: Lane 1: Stomach (Rat) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: SiHa (Human) Cell Lysate at 30 ug Lane 4: DU145 (Human) Cell Lysate at 30 ug Lane 5: RAW264.7 (Mouse) Cell Lysate at 30 ug Lane 6: Liver (Mouse) Lysate at 40 ug Primary: Anti-UCP-2 (bs-1926R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-2) Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (bs-1926R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (bs-1926R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-UCP-2 antibody (bs-1926R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.