| 产品编号 | bs-1903R |
| 英文名称 | Rabbit Anti-Stra8 antibody |
| 中文名称 | 视黄酸激活基因8抗体 |
| 别 名 | Stimulated by retinoic acid Gene 8; STRA8_HUMAN; Stimulated by retinoic acid gene 8 protein homolog; STRA 8; STRA8. |
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Specific References (5) | bs-1903R has been referenced in 5 publications.
[IF=6.582] Changliang Hou. et al. Stella Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation. INT J BIOL SCI. 2022; 18(7): 3006–3018 WB ; Mouse.
[IF=5.048] Yuling Tang. et al. Transcriptomic Profiling of Mouse Mast Cells upon Pathogenic Avian H5N1 and Pandemic H1N1 Influenza a Virus Infection. Viruses-Basel. 2022 Feb;14(2):292 WB ; Mouse.
[IF=4.221] Jinxia Zhai. et al. BDE-209 induce spermatocytes arrest at early-pachytene stage during meiotic prophase I in mice. Toxicology. 2022 Feb;467:153061 WB,IF ; Mouse.
[IF=2.959] Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells.(2018) J. Cell. Biochem. 2018 Oct 18 WB ; Mouse.
[IF=0] Wenhuan Chai. et al. Visualizing Cathepsin K-Cre Expression at the Single-Cell Level with GFP Reporters. JBMR Plus. 2022 Dec;:e10706 IF ; Mouse.
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| 研究领域 | 细胞生物 发育生物学 细胞凋亡 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Stra8: 201-300/330 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a retinoic acid-responsive protein. A homologous protein in mouse has been shown to be involved in the regulation of meiotic initiation in both spermatogenesis and oogenesis, though feature differences between the mouse and human proteins suggest that these homologs are not entirely functionally equivalent. It is thought that this gene may play a role in spermatogenesis in humans. [provided by RefSeq, Nov 2010] Function: Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase Subcellular Location: Cytoplasm. Tissue Specificity: Expressed specifically in testis. Post-translational modifications: Phosphorylated. SWISS: Q7Z7C7 Gene ID: 346673 Database links: Entrez Gene: 346673 Human Entrez Gene: 20899 Mouse Omim: 609987 Human SwissProt: Q7Z7C7 Human SwissProt: P70278 Mouse Unigene: 592279 Human Stra8是哺乳动物生殖细胞由有丝分裂转变为减数分裂前特异表达的基因，是一种生殖细胞特异性表达并受视黄酸诱导的基因。Stra8是减数分裂起始的调节蛋白,参与了雌性胚胎期和雄性出生后生殖细胞的减数分裂起始过程。 |
| 产品图片 |
Sample:
Testis (Rat) Lysate at 40 ug
Primary: Anti-bs-1903R (bs-1903R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-bs-1903R (bs-1903R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stra8) Polyclonal Antibody, Unconjugated (bs-1903R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
