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Rabbit Anti-Serotonin transporter  antibody (bs-1893R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-1893R
英文名称 Serotonin transporter
中文名称 5-羟色胺转运蛋白抗体
别    名 5HT transporter; 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1; SC6A4_HUMAN; SERT; SLC6A4; Sodium dependent serotonin transporter; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; sodium-dependent serotonin transporter; 5-hydroxytryptamine transporter; 5HT transporter; Na+/Cl- dependent serotonin transporter.  
Specific References  (7)     |     bs-1893R has been referenced in 7 publications.
[IF=5.561] Lei Wu. et al. Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway. FOODS. 2023 Jan;12(5):1101  WB ;  Rat.  
[IF=3.693] Huang X et al. The development of the serotonergic and dopaminergic systems during chicken mid-late embryogenesis. Mol Cell Endocrinol. 2019 Aug 1;493:110472.  IHF&WB ;  Hen(egg).  
[IF=3.3] Wojtal, Kacper Andrzej, et al. "Down-regulation of duodenal SLC transporters and activation of pro-inflammatory signaling constitute the early response to high altitude in humans." American Journal of Physiology-Gastrointestinal and Liver Physiology (2014): ajpgi-00353.  WB ;  Human.  
[IF=2.75] Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology (2013).  WB ;  Rat.  
[IF=2.43] Yu, Feng-Yan, et al. "Comparison of 5-hydroxytryptophan signaling pathway characteristics in diarrhea-predominant irritable bowel syndrome and ulcerative colitis." World Journal of Gastroenterology 22.12 (2016): 3451-3459.  IHC-P ;  Human.  
[IF=2.35] Bai, Yang, et al. "4-Chloro-DL-phenylalanine protects against monocrotaline‑induced pulmonary vascular remodeling and lung inflammation." International journal of molecular medicine 33.2 (2014): 373-382.  WB, IHC-P ;  Rat.  
[IF=1.55] Wang, Yun, et al. "Fluoxetine protects against methamphetamine‑induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats." Molecular Medicine Reports 15.2 (2017): 673-680.  WB ;  Rat.  
研究领域 肿瘤  细胞生物  神经生物学  信号转导  通道蛋白  细胞膜受体  药物及化合物  转运蛋白  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Sheep,GuineaPig)
产品应用 WB=1:500-2000, IHC-P=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 70kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human 5-HTT: 401-500/630 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008].

Function:
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

Subunit:
Monomer or homooligomer. Interacts with TGFB1I1. Interacts (via sialylated form) with MYH9. Interacts with SEC23A, SEC24C and INADL. Interacts with NOS1; the interaction may diminish the cell surface localization of SERT in the brain and, correspondingly, reduce serotonin reuptake. Interacts with filamentous actin and STX1A (By similarity). Interacts (via C-terminus) with VIM. Interacts (via C-terminus) with SCAMP2; the interaction is direct and retains transporter molecules intracellularly. Interacts with RAB4 (GTP-bound form); the interaction retains transporter molecules intracellularly.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in platelets (at protein level).

Post-translational modifications:
Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms (By similarity).
Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation (By similarity). Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity.

Similarity:
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

SWISS:
P31645

Gene ID:
6532

Database links:

Entrez Gene: 6532 Human

Entrez Gene: 15567 Mouse

Omim: 182138 Human

SwissProt: P31645 Human

SwissProt: Q60857 Mouse

Unigene: 134662 Human

Unigene: 300318 Mouse



SERT是一种钠依赖单胺转运蛋白,由630个氨基酸组成,相对分子质量为:70KDa
产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SLC6A4 (bs-1893R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (bs-1893R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (bs-1893R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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